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Ageratum enation virus Infection Induces Programmed Cell Death and Alters Metabolite Biosynthesis in Papaver somniferum.
Srivastava, Ashish; Agrawal, Lalit; Raj, Rashmi; Jaidi, Meraj; Raj, Shri K; Gupta, Swati; Dixit, Ritu; Singh, Poonam C; Tripathi, Tusha; Sidhu, Om P; Singh, Brahma N; Shukla, Sudhir; Chauhan, Puneet S; Kumar, Susheel.
Afiliación
  • Srivastava A; Plant Molecular Virology Laboratory, Council of Scientific and Industrial Research - National Botanical Research InstituteLucknow, India.
  • Agrawal L; Amity Institute of Virology and Immunology, Amity UniversityNoida, India.
  • Raj R; Division of Plant Microbe Interaction, Council of Scientific and Industrial Research - National Botanical Research InstituteLucknow, India.
  • Jaidi M; Plant Molecular Virology Laboratory, Council of Scientific and Industrial Research - National Botanical Research InstituteLucknow, India.
  • Raj SK; Plant Molecular Virology Laboratory, Council of Scientific and Industrial Research - National Botanical Research InstituteLucknow, India.
  • Gupta S; Plant Molecular Virology Laboratory, Council of Scientific and Industrial Research - National Botanical Research InstituteLucknow, India.
  • Dixit R; Division of Plant Microbe Interaction, Council of Scientific and Industrial Research - National Botanical Research InstituteLucknow, India.
  • Singh PC; Division of Plant Microbe Interaction, Council of Scientific and Industrial Research - National Botanical Research InstituteLucknow, India.
  • Tripathi T; Division of Plant Microbe Interaction, Council of Scientific and Industrial Research - National Botanical Research InstituteLucknow, India.
  • Sidhu OP; Division of Phytochemistry, Council of Scientific and Industrial Research - National Botanical Research InstituteLucknow, India.
  • Singh BN; Division of Phytochemistry, Council of Scientific and Industrial Research - National Botanical Research InstituteLucknow, India.
  • Shukla S; Division of Pharmacognosy and Ethnopharmacology, Council of Scientific and Industrial Research - National Botanical Research InstituteLucknow, India.
  • Chauhan PS; Plant Breeding Laboratory, Council of Scientific and Industrial Research - National Botanical Research InstituteLucknow, India.
  • Kumar S; Division of Plant Microbe Interaction, Council of Scientific and Industrial Research - National Botanical Research InstituteLucknow, India.
Front Plant Sci ; 8: 1172, 2017.
Article en En | MEDLINE | ID: mdl-28729873
A previously unknown disease which causes severe vein thickening and inward leaf curl was observed in a number of opium poppy (Papaver somniferum L.) plants. The sequence analysis of full-length viral genome and associated betasatellite reveals the occurrence of Ageratum enation virus (AEV) and Ageratum leaf curl betasatellite (ALCB), respectively. Co-infiltration of cloned agroinfectious DNAs of AEV and ALCB induces the leaf curl and vein thickening symptoms as were observed naturally. Infectivity assay confirmed this complex as the cause of disease and also satisfied the Koch's postulates. Comprehensive microscopic analysis of infiltrated plants reveals severe structural anomalies in leaf and stem tissues represented by unorganized cell architecture and vascular bundles. Moreover, the characteristic blebs and membranous vesicles formed due to the virus-induced disintegration of the plasma membrane and intracellular organelles were also present. An accelerated nuclear DNA fragmentation was observed by Comet assay and confirmed by TUNEL and Hoechst dye staining assays suggesting virus-induced programmed cell death. Virus-infection altered the biosynthesis of several important metabolites. The biosynthesis potential of morphine, thebaine, codeine, and papaverine alkaloids reduced significantly in infected plants except for noscapine whose biosynthesis was comparatively enhanced. The expression analysis of corresponding alkaloid pathway genes by real time-PCR corroborated well with the results of HPLC analysis for alkaloid perturbations. The changes in the metabolite and alkaloid contents affect the commercial value of the poppy plants.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Plant Sci Año: 2017 Tipo del documento: Article País de afiliación: India Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Plant Sci Año: 2017 Tipo del documento: Article País de afiliación: India Pais de publicación: Suiza