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X-Ray Phase Contrast Tomography Reveals Early Vascular Alterations and Neuronal Loss in a Multiple Sclerosis Model.
Cedola, A; Bravin, A; Bukreeva, I; Fratini, M; Pacureanu, A; Mittone, A; Massimi, L; Cloetens, P; Coan, P; Campi, G; Spanò, R; Brun, F; Grigoryev, V; Petrosino, V; Venturi, C; Mastrogiacomo, M; Kerlero de Rosbo, Nicole; Uccelli, A.
Afiliación
  • Cedola A; Institute of Nanotechnology- CNR, Rome Unit, Rome, Italy. alessia.cedola@cnr.it.
  • Bravin A; European Synchrotron Radiation Facility, Grenoble, Cedex 9, France.
  • Bukreeva I; Institute of Nanotechnology- CNR, Rome Unit, Rome, Italy.
  • Fratini M; Institute of Nanotechnology- CNR, Rome Unit, Rome, Italy.
  • Pacureanu A; IRCCS Santa Lucia Foundation, Rome, Italy.
  • Mittone A; European Synchrotron Radiation Facility, Grenoble, Cedex 9, France.
  • Massimi L; European Synchrotron Radiation Facility, Grenoble, Cedex 9, France.
  • Cloetens P; Institute of Nanotechnology- CNR, Rome Unit, Rome, Italy.
  • Coan P; European Synchrotron Radiation Facility, Grenoble, Cedex 9, France.
  • Campi G; European Synchrotron Radiation Facility, Grenoble, Cedex 9, France.
  • Spanò R; Ludwig-Maximilians-Universität, Faculty of Medicine and Department of Physics, München, Germany.
  • Brun F; Institute of Crystallography-CNR, Monterotondo, Rome, Italy.
  • Grigoryev V; Department of Experimental Medicine, University of Genoa & Policlinico San Martino - IST Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
  • Petrosino V; Institute of Nanotechnology- CNR, Rome Unit, Rome, Italy.
  • Venturi C; Moscow Engineering Physics Institute/MEPhI, Moscow, Russia.
  • Mastrogiacomo M; Department of Neurosciences, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health Unit, University of Genoa & AOU San Martino - IST Istituto Nazionale per la Ricerca sul Cancro Genoa, Genoa, Italy.
  • Kerlero de Rosbo N; Department of Neurosciences, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health Unit, University of Genoa & AOU San Martino - IST Istituto Nazionale per la Ricerca sul Cancro Genoa, Genoa, Italy.
  • Uccelli A; Department of Experimental Medicine, University of Genoa & Policlinico San Martino - IST Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.
Sci Rep ; 7(1): 5890, 2017 07 19.
Article en En | MEDLINE | ID: mdl-28724999
The degenerative effects of multiple sclerosis at the level of the vascular and neuronal networks in the central nervous system are currently the object of intensive investigation. Preclinical studies have demonstrated the efficacy of mesenchymal stem cell (MSC) therapy in experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis, but the neuropathology of specific lesions in EAE and the effects of MSC treatment are under debate. Because conventional imaging techniques entail protocols that alter the tissues, limiting the reliability of the results, we have used non-invasive X-ray phase-contrast tomography to obtain an unprecedented direct 3D characterization of EAE lesions at micro-to-nano scales, with simultaneous imaging of the vascular and neuronal networks. We reveal EAE-mediated alterations down to the capillary network. Our findings shed light on how the disease and MSC treatment affect the tissues, and promote X-ray phase-contrast tomography as a powerful tool for studying neurovascular diseases and monitoring advanced therapies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Capilares / Tomografía por Rayos X / Esclerosis Múltiple / Neuronas Tipo de estudio: Guideline / Prognostic_studies Límite: Animals Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Capilares / Tomografía por Rayos X / Esclerosis Múltiple / Neuronas Tipo de estudio: Guideline / Prognostic_studies Límite: Animals Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido