A note on ultra-violet red fluorescence of anaerobic bacteria in vitro.
J Appl Bacteriol
; 60(2): 121-6, 1986 Feb.
Article
en En
| MEDLINE
| ID: mdl-2871006
Anaerobes other than the Bacteroides melaninogenicus group isolated from clinical material produce an ultra-violet red fluorescence when grown under certain conditions in vitro. These organisms include other members of the genus Bacteroides as well as strains of some species of Clostridium, Bifidobacterium and Actinomyces. The major fluorescent pigment was identified as protoporphyrin IX. Factors necessary for the production of fluorescence are the presence of blood or haem and a fermentable carbohydrate during growth on a solid medium. Fluorescence intensity was related to the concentration of blood and fermentable carbohydrate present but was independent of inoculum size. Certain commercially available blood agar bases designed specifically for the isolation of fastidious anaerobes from clinical material which contain added carbohydrate were shown to induce fluorescence in certain organisms. This may lead to the misidentification of some anaerobes as B. melaninogenicus.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Porfirinas
/
Protoporfirinas
/
Bacterias Anaerobias
/
Bacteroides
/
Prevotella melaninogenica
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Appl Bacteriol
Año:
1986
Tipo del documento:
Article
Pais de publicación:
Reino Unido