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Controlled basic fibroblast growth factor release device made of poly(ethyleneglycol) dimethacrylates for creating a subcutaneous neovascular bed for cell transplantation.
Yamada, Shinji; Nagai, Nobuhiro; Saijo, Saaya; Kaji, Hirokazu; Nishizawa, Matsuhiko; Imura, Kozue; Goto, Masafumi; Abe, Toshiaki.
Afiliación
  • Yamada S; Division of Clinical Cell Therapy, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan.
  • Nagai N; Division of Clinical Cell Therapy, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan.
  • Saijo S; Division of Clinical Cell Therapy, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan.
  • Kaji H; Department of Finemechanics, Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki, Aoba-ku, Sendai, 980-8579, Japan.
  • Nishizawa M; Department of Finemechanics, Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki, Aoba-ku, Sendai, 980-8579, Japan.
  • Imura K; Division of Transplantation and Regenerative Medicine, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai, 980-0872, Japan.
  • Goto M; Division of Transplantation and Regenerative Medicine, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai, 980-0872, Japan.
  • Abe T; Division of Clinical Cell Therapy, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan.
J Biomed Mater Res A ; 105(11): 3017-3024, 2017 Nov.
Article en En | MEDLINE | ID: mdl-28707305
Subcutaneous space is a potential site for the transplantation of cells such as islets for treatment of type 1 diabetes. To enhance engraftment, an optimal space for the growth of the transplanted cells is needed along with neovascularization. In this study, we developed a device using a photocurable resin, poly(ethyleneglycol) dimethacrylates (PEGDM), for controlled release of basic fibroblast growth factor (bFGF) to create a subcutaneous neovascular bed in rats. The device consists of a disk-shaped capsule with micropores and is composed of tri(ethyleneglycol) dimethacrylate (TEGDM) and a drug formulation of PEGDM. The release rate was tuned by changing the number of pores and the composition of water and PEGDM in the drug formulation. bFGF released from devices incubated in phosphate-buffered saline (PBS) enhanced the growth of fibroblasts, indicating bioactivity of bFGF after release. Histological evaluation showed a significant increase in the extent of vasculature that was dependent on the amount of bFGF loaded into the device. A perfusion study using fluorescein isothiocyanate dextran 2000 kDa showed linear and capillary staining patterns, indicating potent functional vasculature. In conclusion, the controlled bFGF releasing device could provide a neovascular bed with the required vascularization in the subcutaneous space. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3017-3024, 2017.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polietilenglicoles / Factor 2 de Crecimiento de Fibroblastos / Neovascularización Fisiológica / Tejido Subcutáneo / Preparaciones de Acción Retardada / Metacrilatos Límite: Animals / Humans / Male Idioma: En Revista: J Biomed Mater Res A Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polietilenglicoles / Factor 2 de Crecimiento de Fibroblastos / Neovascularización Fisiológica / Tejido Subcutáneo / Preparaciones de Acción Retardada / Metacrilatos Límite: Animals / Humans / Male Idioma: En Revista: J Biomed Mater Res A Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos