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Quantitative real-time imaging of glutathione.
Jiang, Xiqian; Chen, Jianwei; Bajic, Aleksandar; Zhang, Chengwei; Song, Xianzhou; Carroll, Shaina L; Cai, Zhao-Lin; Tang, Meiling; Xue, Mingshan; Cheng, Ninghui; Schaaf, Christian P; Li, Feng; MacKenzie, Kevin R; Ferreon, Allan Chris M; Xia, Fan; Wang, Meng C; Maletic-Savatic, Mirjana; Wang, Jin.
Afiliación
  • Jiang X; Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
  • Chen J; Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
  • Bajic A; Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA.
  • Zhang C; Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, Texas 77030, USA.
  • Song X; Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
  • Carroll SL; Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
  • Cai ZL; Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
  • Tang M; Department of Chemistry, Rice University, Houston, Texas 77030, USA.
  • Xue M; Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA.
  • Cheng N; The Cain Foundation Laboratories, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, Texas 77030, USA.
  • Schaaf CP; Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
  • Li F; Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA.
  • MacKenzie KR; The Cain Foundation Laboratories, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, Texas 77030, USA.
  • Ferreon ACM; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
  • Xia F; Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA.
  • Wang MC; USDA/ARS Children Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA.
  • Maletic-Savatic M; Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, Texas 77030, USA.
  • Wang J; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Nat Commun ; 8: 16087, 2017 07 13.
Article en En | MEDLINE | ID: mdl-28703127
Glutathione plays many important roles in biological processes; however, the dynamic changes of glutathione concentrations in living cells remain largely unknown. Here, we report a reversible reaction-based fluorescent probe-designated as RealThiol (RT)-that can quantitatively monitor the real-time glutathione dynamics in living cells. Using RT, we observe enhanced antioxidant capability of activated neurons and dynamic glutathione changes during ferroptosis. RT is thus a versatile tool that can be used for both confocal microscopy and flow cytometry based high-throughput quantification of glutathione levels in single cells. We envision that this new glutathione probe will enable opportunities to study glutathione dynamics and transportation and expand our understanding of the physiological and pathological roles of glutathione in living cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colorantes Fluorescentes / Fluorometría / Glutatión Tipo de estudio: Evaluation_studies Límite: Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colorantes Fluorescentes / Fluorometría / Glutatión Tipo de estudio: Evaluation_studies Límite: Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido