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BRG1 promotes hepatocarcinogenesis by regulating proliferation and invasiveness.
Kaufmann, Benedikt; Wang, Baocai; Zhong, Suyang; Laschinger, Melanie; Patil, Pranali; Lu, Miao; Assfalg, Volker; Cheng, Zhangjun; Friess, Helmut; Hüser, Norbert; von Figura, Guido; Hartmann, Daniel.
Afiliación
  • Kaufmann B; Department of Surgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
  • Wang B; Department of Surgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
  • Zhong S; II. Medizinische Klinik und Poliklinik, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
  • Laschinger M; Department of Surgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
  • Patil P; Department of Surgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
  • Lu M; Department of General Surgery, the Affiliated Zhongda Hospital, Southeast University, Nanjing, China.
  • Assfalg V; Department of Surgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
  • Cheng Z; Department of General Surgery, the Affiliated Zhongda Hospital, Southeast University, Nanjing, China.
  • Friess H; Department of Surgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
  • Hüser N; Department of Surgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
  • von Figura G; II. Medizinische Klinik und Poliklinik, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
  • Hartmann D; Department of Surgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
PLoS One ; 12(7): e0180225, 2017.
Article en En | MEDLINE | ID: mdl-28700662
The chromatin remodeler complex SWI/SNF plays an important role in physiological and pathological processes. Brahma related gene 1(BRG1), a catalytic subunit of the SWI/SNF complex, is known to be mutated in hepatocellular carcinoma (HCC). However, its role in HCC remains unclear. Here, we investigate the role of BRG1 on cell growth and invasiveness as well as its effect on the expression of putative target genes. Expression of BRG1 was examined in human liver tissue samples and in HCC cell lines. In addition, BRG1 was silenced in human HCC cell lines to analyse cell growth and invasiveness by growth curves, colony formation assay, invasion assay and the expression of putative target genes. BRG1 was found to be significantly increased in HCC samples compared to non-HCC samples. In addition, a declined proliferation rate of BRG1-silenced human HCC cell lines was associated with a decrease of expression of cyclin family members. In line with a decreased invasiveness of BRG1-siRNA-treated human HCC cell lines, down-regulation of MMP7 was detected. These results support the hypothesis that overexpression of BRG1 increases cell growth and invasiveness in HCC. Furthermore, the data highlight cyclin B, E and MMP7 to be associated with BRG1 during hepatocarcinogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Proteínas Nucleares / ADN Helicasas / Carcinoma Hepatocelular / Proliferación Celular / Neoplasias Hepáticas Límite: Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2017 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Proteínas Nucleares / ADN Helicasas / Carcinoma Hepatocelular / Proliferación Celular / Neoplasias Hepáticas Límite: Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2017 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos