Your browser doesn't support javascript.
loading
Molecular characterization and expression analyses of toll like receptor-5 induced by Vibrio parahaemolyticus antigens in Pacific red snapper.
Reyes-Becerril, Martha; Alamillo, Erika; Rosales-Mendoza, Sergio; Ascencio, Felipe; Esteban, M Angeles; Angulo, Carlos.
Afiliación
  • Reyes-Becerril M; Immunology & Vaccinology Group, Centro de Investigaciones Biológicas Del Noroeste (CIBNOR), Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, La Paz B.C.S. 23090, México.
  • Alamillo E; Immunology & Vaccinology Group, Centro de Investigaciones Biológicas Del Noroeste (CIBNOR), Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, La Paz B.C.S. 23090, México.
  • Rosales-Mendoza S; Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, SLP, 78210, México.
  • Ascencio F; Immunology & Vaccinology Group, Centro de Investigaciones Biológicas Del Noroeste (CIBNOR), Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, La Paz B.C.S. 23090, México.
  • Esteban MA; Fish Innate Immune System Group, Department of Cell Biology & Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Spain.
  • Angulo C; Immunology & Vaccinology Group, Centro de Investigaciones Biológicas Del Noroeste (CIBNOR), Instituto Politécnico Nacional 195, Playa Palo de Santa Rita Sur, La Paz B.C.S. 23090, México. Electronic address: eangulo@cibnor.mx.
Fish Shellfish Immunol ; 68: 180-189, 2017 Sep.
Article en En | MEDLINE | ID: mdl-28698126
Toll-like receptor 5 (TLR5) is a member of TLRs family responsible for the bacterial flagellin recognition in vertebrates. Herein, the TLR5M gene structure of Pacific red snapper (Lutjanus peru) was characterized. The full-length cDNA of LpTLR5M comprises an open reading frame (ORF) of 2715 bp, encoding a polypeptide of 904 amino acids including 9 LRRs (residues 119-562) and one LRR-CT domain (residues 593-646) at the extracellular region, and a TIR domain (residues 710-904) in the cytoplasmic region. The amino acid sequence in L. peru TLR5 showed high identity (66-69%) with TLR5 from Paralichthys olivaceus and Scophthalmus maximus. Quantitative real-time PCR (qPCR) analysis demonstrated the constitutive expression of LpTLR5M mRNA in all the examined tissues, with higher levels in intestine, liver, and head-kidney. Furthermore, expression of LpTLR5M and five cytokine genes was also investigated 24 h and one week post-stimulation in fish intraperitoneally injected with ToxA, live V. parahaemolyticus (Vp) or V. parahaemolyticus Lysate antigens. TLR5M was significantly induced in fish infected with Vp. The pro-inflammatory cytokines IL-6, IL8 and IL-12 were significantly up-regulated in head-kidney in fish stimulated with Vp, while in intestine upregulation was observed following ToxA or Lysate injection. In contrast, IL-17 mRNA was significantly up-regulated in the intestine from fish infected with live Vp at 24 h post-injection. The results indicate that Lysate and Vp antigens can induce an immune response via TLR5M and that cytokines have an important role in the defense mechanisms against V. parahaemolyticus.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Perciformes / Regulación de la Expresión Génica / Receptor Toll-Like 5 / Enfermedades de los Peces / Inmunidad Innata Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Perciformes / Regulación de la Expresión Génica / Receptor Toll-Like 5 / Enfermedades de los Peces / Inmunidad Innata Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido