Full-length nisin immunity protein NisI from Lactococcus lactis in a lipid-free form: crystallization and X-ray analysis.
Acta Crystallogr F Struct Biol Commun
; 73(Pt 7): 404-408, 2017 Jul 01.
Article
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| MEDLINE
| ID: mdl-28695849
NisI is a lantibiotic-binding lipoprotein that is specific for nisin. Nisin-producing microorganisms use NisI as an immunity protein for self-protection against nisin. Here, the purification, crystallization and preliminary X-ray diffraction of full-length NisI from Lactobacillus lactis in a lipid-free form (NisI22-C) are reported. Importantly, reductive methylation of the lysine residues in NisI22-C was essential for initial crystallization. Only methylated NisI22-C crystallized. The optimized crystals of methylated NisI22-C were grown in 30-40â
mM ammonium sulfate, 0.1â
M sodium acetate pH 4.6, 16-18% PEG 4000 at 295â
K and diffracted to 1.9â
Å resolution. The crystal belonged to space group P212121, with unit-cell parameters a = 45.99, b = 76.67, c = 76.39â
Å, α = ß = γ = 90.0°. Assuming the presence of one molecule in the asymmetric unit, the estimated Matthews coefficient (VM) is 2.58â
Å3â
Da-1 and the estimated solvent content is 52.3%.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
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Lactococcus lactis
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Lipoproteínas
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Lisina
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Proteínas de la Membrana
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Nisina
Idioma:
En
Revista:
Acta Crystallogr F Struct Biol Commun
Año:
2017
Tipo del documento:
Article
Pais de publicación:
Estados Unidos