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Structural characterisation of a water-soluble polysaccharide from tissue-cultured Dendrobium huoshanense C.Z. Tang et S.J. Cheng.
Si, Hua-Yang; Chen, Nai-Fu; Chen, Nai-Dong; Huang, Cheng; Li, Jun; Wang, Hui.
Afiliación
  • Si HY; a College of Biotechnology and Pharmaceutical Engineering , West Anhui University , Lu'an City , China.
  • Chen NF; b West Anhui Biotechnology Research Center of Natural Medicine and Traditional Chinese Medicine , West Anhui University , Lu'an City , China.
  • Chen ND; c School of Pharmacy , Anhui University of Chinese Medicine , Hefei , China.
  • Huang C; a College of Biotechnology and Pharmaceutical Engineering , West Anhui University , Lu'an City , China.
  • Li J; b West Anhui Biotechnology Research Center of Natural Medicine and Traditional Chinese Medicine , West Anhui University , Lu'an City , China.
  • Wang H; c School of Pharmacy , Anhui University of Chinese Medicine , Hefei , China.
Nat Prod Res ; 32(3): 252-260, 2018 Feb.
Article en En | MEDLINE | ID: mdl-28691858
A water-soluble polysaccharide TC-DHPA4 with a molecular weight of 8.0 × 105 Da was isolated from tissue-cultured Dendrobium huoshanense by anion exchange and gel permeation chromatography. Monosaccharide analysis revealed that the homogeneous polysaccharide was made up of rhamnose, arabinose, mannose, glucose, galactose and glucuronic acid with a molar ratio of 1.28:1:1.67:4.71:10.43:1.42. The sugar residue sequence analysis based on the GC-MS files and NMR spectra indicated that the backbone of TC-DHPA4 consisted of the repeated units:→6)-ß-Galp-(1→6)-ß-Galp-(1→4)-ß-GlcpA-(1→6)-ß-Glcp-(1→6)-ß-Glcp-(→. The sugar residue sequences ß-Glcp-(1→)-α-Rhap-(1→3)-ß-Galp-(1→, ß-Glcp-(1→4)-α-Rhap-(1→3)-ß-Galp-(1→, ß-Galp-(1→6)-ß-Manp-(1→3)-ß-Galp-(1→, and α-l-Araf-(1→2)-ß-Manp-(1→3)-ß-Galp-(1→ were identified as the branches attached to the C-3 position of (1→6)-linked galactose in the backbone.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Dendrobium / Monosacáridos Idioma: En Revista: Nat Prod Res Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Dendrobium / Monosacáridos Idioma: En Revista: Nat Prod Res Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido