Unexpected features of exponentially growing Tobacco Bright Yellow-2 cell suspension culture in relation to excreted extracellular polysaccharides and cell wall composition.

Issawi, Mohammad; Muhieddine, Mohammad; Girard, Celine; Sol, Vincent; Riou, Catherine

Issawi, Mohammad; Muhieddine, Mohammad; Girard, Celine; Sol, Vincent; Riou, Catherine.

Afiliación

Issawi M; Laboratoire de Chimie des Substances Naturelles, Université de Limoges, EA 1069, 123 avenue Albert Thomas, 87060, Limoges, France.
Muhieddine M; Laboratoire de Chimie des Substances Naturelles, Université de Limoges, EA 1069, 123 avenue Albert Thomas, 87060, Limoges, France.
Girard C; Laboratoire de Chimie des Substances Naturelles, Université de Limoges, EA 1069, 123 avenue Albert Thomas, 87060, Limoges, France.
Sol V; Laboratoire de Chimie des Substances Naturelles, Université de Limoges, EA 1069, 123 avenue Albert Thomas, 87060, Limoges, France.
Riou C; Laboratoire de Chimie des Substances Naturelles, Université de Limoges, EA 1069, 123 avenue Albert Thomas, 87060, Limoges, France. catherine.riou@unilim.fr.

Glycoconj J ; 34(5): 585-590, 2017 10.

Article en En | MEDLINE | ID: mdl-28676909

RESUMEN

This article presents a new insight about TBY-2 cells; from extracellular polysaccharides secretion to cell wall composition during cell suspension culture. In the medium of cells taken 2 days after dilution (end of lag phase), a two unit pH decrease from 5.38 to 3.45 was observed and linked to a high uronic acid (UA) amount secretion (47.8%) while, in 4 and 7 day-old spent media, pH increased and UA amounts decreased 35.6 and 42.3% UA, respectively. To attain deeper knowledge of the putative link between extracellular polysaccharide excretion and cell wall composition, we determined cell wall UA and neutral sugar composition of cells from D2 to D12 cultures. While cell walls from D2 and D3 cells contained a large amount of uronic acid (twice as much as the other analysed cell walls), similar amounts of neutral sugar were detected in cells from lag to end of exponential phase cells suggesting an enriched pectin network in young cultures. Indeed, monosaccharide composition analysis leads to an estimated percentage of pectins of 56% for D3 cell wall against 45% D7 cell walls indicating that the cells at the mid-exponential growth phase re-organized their cell wall linked to a decrease in secreted UA that finally led to a stabilization of the spent medium pH to 5.4. In conclusion, TBY-2 cell suspension from lag to stationary phase showed cell wall remodeling that could be of interest in drug interaction and internalization study.

Asunto(s)

Pared Celular/química; Nicotiana/metabolismo; Células Vegetales/química; Polisacáridos/aislamiento & purificación; Ácidos Urónicos/aislamiento & purificación; Técnicas de Cultivo de Célula; Pared Celular/metabolismo; Células Cultivadas; Concentración de Iones de Hidrógeno; Monosacáridos/aislamiento & purificación; Monosacáridos/metabolismo; Pectinas/aislamiento & purificación; Pectinas/metabolismo; Células Vegetales/metabolismo; Polisacáridos/metabolismo; Nicotiana/citología; Nicotiana/crecimiento & desarrollo; Ácidos Urónicos/metabolismo

Palabras clave

Cell wall composition; Extracellular polysaccharides; Tobacco BY-2 suspension culture; Uronic acid

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Nicotiana / Ácidos Urónicos / Pared Celular / Células Vegetales Idioma: En Revista: Glycoconj J Asunto de la revista: BIOQUIMICA / METABOLISMO Año: 2017 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos

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