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The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum.
Hon, Shuen; Olson, Daniel G; Holwerda, Evert K; Lanahan, Anthony A; Murphy, Sean J L; Maloney, Marybeth I; Zheng, Tianyong; Papanek, Beth; Guss, Adam M; Lynd, Lee R.
Afiliación
  • Hon S; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA.
  • Olson DG; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA. Electronic address: Daniel.G.Olson@dartmouth.edu.
  • Holwerda EK; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA.
  • Lanahan AA; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA.
  • Murphy SJL; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA.
  • Maloney MI; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA.
  • Zheng T; Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA.
  • Papanek B; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA.
  • Guss AM; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA.
  • Lynd LR; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA; Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA; Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA. Electronic address: Lee.R.Lynd@dartmouth.edu.
Metab Eng ; 42: 175-184, 2017 07.
Article en En | MEDLINE | ID: mdl-28663138
Clostridium thermocellum ferments cellulose, is a promising candidate for ethanol production from cellulosic biomass, and has been the focus of studies aimed at improving ethanol yield. Thermoanaerobacterium saccharolyticum ferments hemicellulose, but not cellulose, and has been engineered to produce ethanol at high yield and titer. Recent research has led to the identification of four genes in T. saccharolyticum involved in ethanol production: adhE, nfnA, nfnB and adhA. We introduced these genes into C. thermocellum and observed significant improvements to ethanol yield, titer, and productivity. The four genes alone, however, were insufficient to achieve in C. thermocellum the ethanol yields and titers observed in engineered T. saccharolyticum strains, even when combined with gene deletions targeting hydrogen production. This suggests that other parts of T. saccharolyticum metabolism may also be necessary to reproduce the high ethanol yield and titer phenotype in C. thermocellum.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Clostridium thermocellum / Thermoanaerobacterium / Etanol Idioma: En Revista: Metab Eng Asunto de la revista: ENGENHARIA BIOMEDICA / METABOLISMO Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Bélgica
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Clostridium thermocellum / Thermoanaerobacterium / Etanol Idioma: En Revista: Metab Eng Asunto de la revista: ENGENHARIA BIOMEDICA / METABOLISMO Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Bélgica