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Activating transcription factor 3 protects mice against pseudomonas aeruginosa-induced acute lung injury by interacting with lipopolysaccharide binding protein.
Zhao, Yunfeng; Wu, Xiulin; Qian, Lanlan; Guo, Liang; Liao, Jiangrong; Wu, Xueling.
Afiliación
  • Zhao Y; Department of Respiratory Medicine, Punan hospital of Pudong New District, Shanghai, China.
  • Wu X; Department of Respiratory Medicine, Chongqing Armed Police Hospital, Chongqing, China.
  • Qian L; Department of Respiratory Medicine, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
  • Guo L; Department of Respiratory Medicine, Xinqiao Hospital, Third Military Medical University, Chongqing, China.
  • Liao J; Institute of Respiratory Medicine, Guizhou Aerospace Hospital, Zunyi, China.
  • Wu X; Department of Respiratory Medicine, Renji Hospital, School of medicine, Shanghai Jiaotong University, 160, Pujian Road, Shanghai, 200127, China. Electronic address: wuxueling76@126.com.
Mol Immunol ; 90: 27-32, 2017 10.
Article en En | MEDLINE | ID: mdl-28662411
Excessive inflammatory response is critical event in the pathogenesis of acute lung injury (ALI). Previous study has shown that activating transcription factor 3 (ATF3) plays a role in downregulate inflammatory responses including ventilation-induced ALI. We hypothesized that ATF3 have a protective effect in ALI induced by pseudomonas aeruginosa. PA was intra-tracheally administrated to ATF3 knock-out (KO) mice to establish ALI model. Inflammatory factors, BALF protein, lung wet to dry ratio, lung injury score and mortality were determined. The activation of NF-κB was detected by western blot and Co-immunoprecipitation (Co-ip) was used to determinate the binding of ATF3 to LBP. Peritoneal macrophages were isolated from ATF3 KO mice and stimulated by PA. PA increased the expression of ATF3 in the lung tissues in ATF3 wild type (WT) mice. ATF3 deficiency significantly increased the concentration of TNFα, IL-6 and IL-1ß in the supernatant of peritoneal macrophages, lung tissue and BALF after PA stimulation and also enhanced the activity of NF-κB. ATF3 deficiency also enhanced the BALF protein concentration and increased the lung wet to dry ratio. The lung injury score and mortality were higher in ATF3 KO mice treated with PA. Moreover, ATF3 was demonstrated to bind to LBP These finding suggest ATF3 protect mice against ALI induced by PA partly due to the binding to LBP.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Pseudomonas / Proteínas de Fase Aguda / Glicoproteínas de Membrana / Proteínas Portadoras / Macrófagos Peritoneales / Factor de Transcripción Activador 3 / Lesión Pulmonar Aguda Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Mol Immunol Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Pseudomonas / Proteínas de Fase Aguda / Glicoproteínas de Membrana / Proteínas Portadoras / Macrófagos Peritoneales / Factor de Transcripción Activador 3 / Lesión Pulmonar Aguda Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Mol Immunol Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido