Your browser doesn't support javascript.
loading
RNase III nucleases from diverse kingdoms serve as antiviral effectors.
Aguado, Lauren C; Schmid, Sonja; May, Jared; Sabin, Leah R; Panis, Maryline; Blanco-Melo, Daniel; Shim, Jaehee V; Sachs, David; Cherry, Sara; Simon, Anne E; Levraud, Jean-Pierre; tenOever, Benjamin R.
Afiliación
  • Aguado LC; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
  • Schmid S; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
  • May J; Department of Cell Biology and Molecular Genetics, University of Maryland College Park, College Park, Maryland 20742, USA.
  • Sabin LR; Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
  • Panis M; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
  • Blanco-Melo D; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
  • Shim JV; Department of Pharmacology and Therapeutics, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
  • Sachs D; Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
  • Cherry S; Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
  • Simon AE; Department of Cell Biology and Molecular Genetics, University of Maryland College Park, College Park, Maryland 20742, USA.
  • Levraud JP; Macrophages et Développement de l'Immunité, Institut Pasteur, CNRS UMR3738, 25-28 rue du Dr. Roux, 75724 Paris Cedex 15, France.
  • tenOever BR; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York 10029, USA.
Nature ; 547(7661): 114-117, 2017 07 06.
Article en En | MEDLINE | ID: mdl-28658212
In contrast to the DNA-based viruses in prokaryotes, the emergence of eukaryotes provided the necessary compartmentalization and membranous environment for RNA viruses to flourish, creating the need for an RNA-targeting antiviral system. Present day eukaryotes employ at least two main defence strategies that emerged as a result of this viral shift, namely antiviral RNA interference and the interferon system. Here we demonstrate that Drosha and related RNase III ribonucleases from all three domains of life also elicit a unique RNA-targeting antiviral activity. Systemic evolution of ligands by exponential enrichment of this class of proteins illustrates the recognition of unbranched RNA stem loops. Biochemical analyses reveal that, in this context, Drosha functions as an antiviral clamp, conferring steric hindrance on the RNA-dependent RNA polymerases of diverse positive-stranded RNA viruses. We present evidence for cytoplasmic translocation of RNase III nucleases in response to virus in diverse eukaryotes including plants, arthropods, fish, and mammals. These data implicate RNase III recognition of viral RNA as an antiviral defence that is independent of, and possibly predates, other known eukaryotic antiviral systems.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antivirales / Virus ARN / Evolución Molecular / Ribonucleasa III Límite: Animals / Humans Idioma: En Revista: Nature Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antivirales / Virus ARN / Evolución Molecular / Ribonucleasa III Límite: Animals / Humans Idioma: En Revista: Nature Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido