Your browser doesn't support javascript.
loading
DNA cytoskeleton for stabilizing artificial cells.
Kurokawa, Chikako; Fujiwara, Kei; Morita, Masamune; Kawamata, Ibuki; Kawagishi, Yui; Sakai, Atsushi; Murayama, Yoshihiro; Nomura, Shin-Ichiro M; Murata, Satoshi; Takinoue, Masahiro; Yanagisawa, Miho.
Afiliación
  • Kurokawa C; Department of Applied Physics, Tokyo University of Agriculture and Technology, Tokyo 184-8588 Japan.
  • Fujiwara K; Department of Biosciences and Informatics, Keio University, Kanagawa 223-8522, Japan.
  • Morita M; Department of Computer Science, Tokyo Institute of Technology, Kanagawa 226-8502, Japan.
  • Kawamata I; Department of Robotics, Tohoku University, Sendai 980-8579, Japan.
  • Kawagishi Y; Department of Robotics, Tohoku University, Sendai 980-8579, Japan.
  • Sakai A; Department of Applied Physics, Tokyo University of Agriculture and Technology, Tokyo 184-8588 Japan.
  • Murayama Y; Department of Applied Physics, Tokyo University of Agriculture and Technology, Tokyo 184-8588 Japan.
  • Nomura SM; Department of Robotics, Tohoku University, Sendai 980-8579, Japan.
  • Murata S; Department of Robotics, Tohoku University, Sendai 980-8579, Japan.
  • Takinoue M; Department of Computer Science, Tokyo Institute of Technology, Kanagawa 226-8502, Japan; myanagi@cc.tuat.ac.jp takinoue@c.titech.ac.jp.
  • Yanagisawa M; Department of Applied Physics, Tokyo University of Agriculture and Technology, Tokyo 184-8588 Japan; myanagi@cc.tuat.ac.jp takinoue@c.titech.ac.jp.
Proc Natl Acad Sci U S A ; 114(28): 7228-7233, 2017 07 11.
Article en En | MEDLINE | ID: mdl-28652345
Cell-sized liposomes and droplets coated with lipid layers have been used as platforms for understanding live cells, constructing artificial cells, and implementing functional biomedical tools such as biosensing platforms and drug delivery systems. However, these systems are very fragile, which results from the absence of cytoskeletons in these systems. Here, we construct an artificial cytoskeleton using DNA nanostructures. The designed DNA oligomers form a Y-shaped nanostructure and connect to each other with their complementary sticky ends to form networks. To undercoat lipid membranes with this DNA network, we used cationic lipids that attract negatively charged DNA. By encapsulating the DNA into the droplets, we successfully created a DNA shell underneath the membrane. The DNA shells increased interfacial tension, elastic modulus, and shear modulus of the droplet surface, consequently stabilizing the lipid droplets. Such drastic changes in stability were detected only when the DNA shell was in the gel phase. Furthermore, we demonstrate that liposomes with the DNA gel shell are substantially tolerant against outer osmotic shock. These results clearly show the DNA gel shell is a stabilizer of the lipid membrane akin to the cytoskeleton in live cells.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citoesqueleto / ADN / Lípidos / Liposomas Límite: Humans Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citoesqueleto / ADN / Lípidos / Liposomas Límite: Humans Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos