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Opposing activities of oncogenic MIR17HG and tumor suppressive MIR100HG clusters and their gene targets regulate replicative senescence in human adult stem cells.
Lopez, Mary F; Niu, Ping; Wang, Lu; Vogelsang, Maryann; Gaur, Meenakshi; Krastins, Bryan; Zhao, Yueqiang; Smagul, Aibek; Nussupbekova, Aliya; Akanov, Aikan A; Jordan, I King; Lunyak, Victoria V.
Afiliación
  • Lopez MF; Nuclea Biotechnologies, Cambridge, MA 02140 UK.
  • Niu P; Department of Pediatrics, Renmin Hospital of Wuhan University, Wuhan, 430060 China.
  • Wang L; Aelan Cell Technology, San Francisco, CA 92107 USA.
  • Vogelsang M; School of Biology, Georgia Institute of Technology, Atlanta, GA 30306 USA.
  • Gaur M; Nuclea Biotechnologies, Cambridge, MA 02140 UK.
  • Krastins B; Aelan Cell Technology, San Francisco, CA 92107 USA.
  • Zhao Y; Nuclea Biotechnologies, Cambridge, MA 02140 UK.
  • Smagul A; Department of Plastic Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060 China.
  • Nussupbekova A; Kazakh Medical University, Almaty, Kazakhstan.
  • Akanov AA; Aelan Cell Technology, San Francisco, CA 92107 USA.
  • Jordan IK; Kazakh Medical University, Almaty, Kazakhstan.
  • Lunyak VV; Kazakh Medical University, Almaty, Kazakhstan.
NPJ Aging Mech Dis ; 3: 7, 2017.
Article en En | MEDLINE | ID: mdl-28649425
Growing evidence suggests that many diseases of aging, including diseases associated with robust changes and adipose deports, may be caused by resident adult stem cell exhaustion due to the process called cellular senescence. Understanding how microRNA pathways can regulate cellular senescence is crucial for the development of novel diagnostic and therapeutic strategies to combat these pathologies. Herein, using integrated transcriptomic and semi-quantitative proteomic analysis, we provide a system level view of the regulation of human adipose-derived stem cell senescence by a subset of mature microRNAs (termed senescence-associated-microRNAs) produced by biogenesis of oncogenic MIR17HG and tumor-suppressive MIR100HG clusters. We demonstrate functional significance of these mature senescence-associated-microRNAs in the process of replicative senescence of human adipose-derived stem cells ex-vivo and define a set of senescence-associated-microRNA gene targets that are able to elicit, modulate and, most importantly, balance intimate connections between oncogenic and senescent events.

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: NPJ Aging Mech Dis Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: NPJ Aging Mech Dis Año: 2017 Tipo del documento: Article Pais de publicación: Reino Unido