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A highly sensitive carbapenemase assay using laser desorption/ionization mass spectrometry based on a parylene-matrix chip.
Park, Jong-Min; Kim, Jo-Il; Noh, Joo-Yoon; Kim, Mira; Kang, Min-Jung; Pyun, Jae-Chul.
Afiliación
  • Park JM; Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu Seoul, 03722, Korea.
  • Kim JI; Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu Seoul, 03722, Korea.
  • Noh JY; Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu Seoul, 03722, Korea.
  • Kim M; Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu Seoul, 03722, Korea.
  • Kang MJ; Korea Institute of Science and Technology (KIST), Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul, 02792, Korea.
  • Pyun JC; Department of Materials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu Seoul, 03722, Korea. Electronic address: jcpyun@yonsei.ac.kr.
Enzyme Microb Technol ; 104: 56-68, 2017 Sep.
Article en En | MEDLINE | ID: mdl-28648181
A quantitative carbapenemase assay was developed using laser desorption/ionization mass spectrometry (LDI-MS) based on a parylene-matrix chip. As a first step, the reproducibility (spot-to-spot, shot-to-shot, and day-to-day) of LDI-MS based on a parylene-matrix chip and the quantification ranges for four carbapenem antibiotics (doripenem, ertapenem, imipenem, and meropenem) were determined. A carbapenem-susceptibility test was performed using the four carbapenems and 51 bacterial strains that displayed (1) carbapenem resistance with carbapenemase, (2) carbapenem resistance without carbapenemase, or (3) carbapenem susceptibility. The susceptibility test results showed that LDI-MS based on a parylene-matrix chip was more sensitive and selective for detecting the carbapenemase reaction than conventional MALDI-TOF MS based on a 2,5-dihydroxybenzoic acid matrix.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Beta-Lactamasas / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción Tipo de estudio: Diagnostic_studies / Evaluation_studies Idioma: En Revista: Enzyme Microb Technol Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Beta-Lactamasas / Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción Tipo de estudio: Diagnostic_studies / Evaluation_studies Idioma: En Revista: Enzyme Microb Technol Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos