Your browser doesn't support javascript.
loading
[Experimental development and rationale for a renal decellularization protocol with subsequent comprehensive assessment of the biological scaffold].
Glybochko, P V; Alekseenko, S N; Gubareva, E A; Kuevda, E V; Basov, A A; Sotnichenko, A S; Dzhimak, S S; Gumenyuk, I S; Egiev, I Kh; Chechelyan, V N; Nakokhov, R Z; Lyasota, O M; Teterin, Yu V.
Afiliación
  • Glybochko PV; I.M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
  • Alekseenko SN; Kuban State Medical University of Minzdrav of Russia, Krasnodar, Russia.
  • Gubareva EA; Kuban State University, Krasnodar, Russia.
  • Kuevda EV; I.M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
  • Basov AA; Kuban State Medical University of Minzdrav of Russia, Krasnodar, Russia.
  • Sotnichenko AS; Kuban State University, Krasnodar, Russia.
  • Dzhimak SS; I.M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
  • Gumenyuk IS; Kuban State Medical University of Minzdrav of Russia, Krasnodar, Russia.
  • Egiev IK; Kuban State University, Krasnodar, Russia.
  • Chechelyan VN; I.M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
  • Nakokhov RZ; Kuban State Medical University of Minzdrav of Russia, Krasnodar, Russia.
  • Lyasota OM; Kuban State University, Krasnodar, Russia.
  • Teterin YV; I.M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
Urologiia ; (2): 5-13, 2017 Jun.
Article en Ru | MEDLINE | ID: mdl-28631900
Chronic renal failure (CRF) is one of the most challenging problems of contemporary medicine. Patients with chronic renal failure usually need renal replacement therapy as either hemodialysis, peritoneal dialysis or a kidney transplant. The latter is the most promising option for end-stage kidney disease. However, the shortage of donor organs, the complexity of their delivery, the difficulty in finding an immunologically compatible donor and the need for lifelong immunosuppression triggered advances in modern tissue engineering. In this field, the primary priority is focused on developing bioengineered scaffolds with subsequent recellularization with autologous cells. Using such constructs would allow for solving both ethical and immunological problems of transplantation. The aim of this pilot study was to develop a new method of renal decellularization using small laboratory animals. MATERIALS AND METHODS: The study investigated the morphological structure of the obtained decellularized matrix and quantitatively tested DNA residues in the resulting scaffold. We proposed a new biophysical method for assessing the matrix quality using the EPR spectroscopy and conducted experiments on the matrix recellularization with mesenchymal multipotent stem cells to estimate cytotoxicity, cell viability and metabolic activity. RESULTS: The obtained decellularized renal matrix retained the native tissue architecture after a complete removal of the cell material, had no cytotoxic properties and supported cell adhesion and proliferation. CONCLUSION: All the above suggests that the proposed decellularization protocol is a promising method to produce tissue-engineered kidney constructs with possible clinical application in the foreseeable future.
Asunto(s)
Palabras clave
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Andamios del Tejido / Riñón / Riñones Artificiales / Fallo Renal Crónico Tipo de estudio: Guideline Aspecto: Ethics Límite: Animals Idioma: Ru Revista: Urologiia Asunto de la revista: UROLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Rusia
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingeniería de Tejidos / Andamios del Tejido / Riñón / Riñones Artificiales / Fallo Renal Crónico Tipo de estudio: Guideline Aspecto: Ethics Límite: Animals Idioma: Ru Revista: Urologiia Asunto de la revista: UROLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Rusia