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Phylogenetic analysis of F-bZIP transcription factors indicates conservation of the zinc deficiency response across land plants.
Castro, Pedro Humberto; Lilay, Grmay H; Muñoz-Mérida, Antonio; Schjoerring, Jan K; Azevedo, Herlânder; Assunção, Ana G L.
Afiliación
  • Castro PH; Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871, Copenhagen, Denmark.
  • Lilay GH; Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871, Copenhagen, Denmark.
  • Muñoz-Mérida A; CIBIO, InBIO - Research Network in Biodiversity and Evolutionary Biology, University of Porto, Campus Agrário de Vairão, 4485-661, Vairão, Portugal.
  • Schjoerring JK; Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871, Copenhagen, Denmark.
  • Azevedo H; CIBIO, InBIO - Research Network in Biodiversity and Evolutionary Biology, University of Porto, Campus Agrário de Vairão, 4485-661, Vairão, Portugal.
  • Assunção AGL; Department of Biology, Faculty of Sciences, University of Porto, Rua Campo Alegre, 4169-007, Porto, Portugal.
Sci Rep ; 7(1): 3806, 2017 06 19.
Article en En | MEDLINE | ID: mdl-28630437
Basic leucine zipper (bZIP) transcription factors control important developmental and physiological processes in plants. In Arabidopsis thaliana, the three gene F-bZIP subfamily has been associated with zinc deficiency and salt stress response. Benefiting from the present abundance of plant genomic data, we performed an evolutionary and structural characterization of plant F-bZIPs. We observed divergence during seed plant evolution, into two groups and inferred different selective pressures for each. Group 1 contains AtbZIP19 and AtbZIP23 and appears more conserved, whereas Group 2, containing AtbZIP24, is more prone to gene loss and expansion events. Transcriptomic and experimental data reinforced AtbZIP19/23 as pivotal regulators of the zinc deficiency response, mostly via the activation of genes from the ZIP metal transporter family, and revealed that they are the main regulatory switch of AtZIP4. A survey of AtZIP4 orthologs promoters across different plant taxa revealed an enrichment of the Zinc Deficiency Response Element (ZDRE) to which both AtbZIP19/23 bind. Overall, our results indicate that while the AtbZIP24 function in the regulation of the salt stress response may be the result of neo-functionalization, the AtbZIP19/23 function in the regulation of the zinc deficiency response may be conserved in land plants (Embryophytes).
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Factores de Transcripción / Zinc / Leucina Zippers / Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Factores de Transcripción / Zinc / Leucina Zippers / Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Reino Unido