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Synthesis and Detection by HPLC of 3-Oxohexadecanoyl-CoA for the Study of Peroxisomal Bifunctional Proteins.
Tsuchida, Shirou; Tenma, Ayumu; Hamaue, Naoya; Murai, Tsuyoshi; Yoshimura, Teruki; Aoki, Takashi; Kurosawa, Takao.
Afiliación
  • Tsuchida S; Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido.
  • Tenma A; Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido.
  • Hamaue N; Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido.
  • Murai T; Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido.
  • Yoshimura T; Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido.
  • Aoki T; Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido.
  • Kurosawa T; Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido.
J Oleo Sci ; 66(7): 745-751, 2017 Jul 01.
Article en En | MEDLINE | ID: mdl-28626134
3-oxohexadecanoyl-CoA was synthesized for the study of D-bifunctional protein (EC 4. 2. 1. 107, EC 4. 2. 1. 119, EC 1. 1. 1. n12) and L-bifunctional protein (EC 4. 2. 1. 17, EC 5. 3. 3. 8, EC 1. 1. 1. 35). First, tetradecanal was subjected to the Reformatsky reaction with ethyl bromoacetate, and the product was then converted into ethyl 3-oxohexadecanoate. After acetalization of the 3-oxo ester with ethylene glycol, 3,3-ethlenedioxyhexadecanoic acid was obtained by alkaline hydrolysis. The acid was condensed with coenzyme A (CoA) by the mixed anhydride method, and the resulting CoA ester was deprotected with 4 M HCl to obtain 3-oxohexadecanoyl-CoA. In addition, the behavior of the CoA ester under several conditions of high-performance liquid chromatography (HPLC) was also investigated. We established separation detection of (R)-3-hydroxyhexadecanoyl-CoA, (S)-3-hydroxyhexadecaboyl-CoA, 3-oxohexadecanoyl-CoA, and trans-2-hexadecenoyl-CoA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acilcoenzima A / Cromatografía Líquida de Alta Presión / Proteína-2 Multifuncional Peroxisomal Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Oleo Sci Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article Pais de publicación: Japón
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acilcoenzima A / Cromatografía Líquida de Alta Presión / Proteína-2 Multifuncional Peroxisomal Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Oleo Sci Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article Pais de publicación: Japón