The Irr and RirA Proteins Participate in a Complex Regulatory Circuit and Act in Concert To Modulate Bacterioferritin Expression in Ensifer meliloti 1021.

Costa, Daniela; Amarelle, Vanesa; Valverde, Claudio; O'Brian, Mark R; Fabiano, Elena

Costa, Daniela; Amarelle, Vanesa; Valverde, Claudio; O'Brian, Mark R; Fabiano, Elena.

Afiliación

Costa D; Biochemical and Microbial Genomics Department, Instituto de Investigaciones Biológicas Clemente Estable, MEC, Montevideo, Uruguay.
Amarelle V; Biochemical and Microbial Genomics Department, Instituto de Investigaciones Biológicas Clemente Estable, MEC, Montevideo, Uruguay.
Valverde C; Universidad Nacional de Quilmes-CONICET, Buenos Aires, Argentina.
O'Brian MR; State University of New York at Buffalo, Buffalo, New York, USA.
Fabiano E; Biochemical and Microbial Genomics Department, Instituto de Investigaciones Biológicas Clemente Estable, MEC, Montevideo, Uruguay efabiano@iibce.edu.uy.

Appl Environ Microbiol ; 83(16)2017 08 15.

Article en En | MEDLINE | ID: mdl-28625986

RESUMEN

In this work we found that the bfr gene of the rhizobial species Ensifer meliloti, encoding a bacterioferritin iron storage protein, is involved in iron homeostasis and the oxidative stress response. This gene is located downstream of and overlapping the smc03787 open reading frame (ORF). No well-predicted RirA or Irr boxes were found in the region immediately upstream of the bfr gene although two presumptive RirA boxes and one presumptive Irr box were present in the putative promoter of smc03787 We demonstrate that bfr gene expression is enhanced under iron-sufficient conditions and that Irr and RirA modulate this expression. The pattern of bfr gene expression as well as the response to Irr and RirA is inversely correlated to that of smc03787 Moreover, our results suggest that the small RNA SmelC759 participates in RirA- and Irr-mediated regulation of bfr expression and that additional unknown factors are involved in iron-dependent regulation.IMPORTANCEE. meliloti belongs to the Alphaproteobacteria, a group of bacteria that includes several species able to associate with eukaryotic hosts, from mammals to plants, in a symbiotic or pathogenic manner. Regulation of iron homeostasis in this group of bacteria differs from that found in the well-studied Gammaproteobacteria In this work we analyzed the effect of rirA and irr mutations on bfr gene expression. We demonstrate the effect of an irr mutation on iron homeostasis in this bacterial genus. Moreover, results obtained indicate a complex regulatory circuit where multiple regulators, including RirA, Irr, the small RNA SmelC759, and still unknown factors, act in concert to balance bfr gene expression.

Asunto(s)

Proteínas Bacterianas/genética; Proteínas Bacterianas/metabolismo; Grupo Citocromo b/genética; Ferritinas/genética; Regulación Bacteriana de la Expresión Génica; Proteínas Reguladoras del Hierro/metabolismo; Hierro/metabolismo; ARN Bacteriano/metabolismo; Sinorhizobium meliloti/metabolismo; Factores de Transcripción/metabolismo; Proteínas Bacterianas/biosíntesis; Grupo Citocromo b/biosíntesis; Ferritinas/biosíntesis; Proteínas Reguladoras del Hierro/genética; Mutación; ARN Bacteriano/genética; Sinorhizobium meliloti/genética; Factores de Transcripción/genética

Palabras clave

Sinorhizobium; bacterioferritin; iron metabolism; iron regulation

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Factores de Transcripción / ARN Bacteriano / Regulación Bacteriana de la Expresión Génica / Sinorhizobium meliloti / Grupo Citocromo b / Proteínas Reguladoras del Hierro / Ferritinas / Hierro Tipo de estudio: Prognostic_studies Idioma: En Revista: Appl Environ Microbiol Año: 2017 Tipo del documento: Article País de afiliación: Uruguay Pais de publicación: Estados Unidos

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