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Gastrointestinal Simulation Model TWIN-SHIME Shows Differences between Human Urolithin-Metabotypes in Gut Microbiota Composition, Pomegranate Polyphenol Metabolism, and Transport along the Intestinal Tract.
García-Villalba, Rocío; Vissenaekens, Hanne; Pitart, Judit; Romo-Vaquero, María; Espín, Juan C; Grootaert, Charlotte; Selma, María V; Raes, Katleen; Smagghe, Guy; Possemiers, Sam; Van Camp, John; Tomas-Barberan, Francisco A.
Afiliación
  • García-Villalba R; Research Group on Quality, Safety, and Bioactivity of Plant Foods, Laboratory of Food & Health, Department of Food Science and Technology, CEBAS-CSIC , 30100 Campus de Espinardo, Murcia Spain.
  • Vissenaekens H; Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University , Ghent, Belgium.
  • Pitart J; Department of Industrial Biological Sciences, Faculty of Bioscience Engineering, Ghent University , Kortrijk, Belgium.
  • Romo-Vaquero M; Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University , Ghent, Belgium.
  • Espín JC; ProDigest BVBA , Ghent, Belgium.
  • Grootaert C; Research Group on Quality, Safety, and Bioactivity of Plant Foods, Laboratory of Food & Health, Department of Food Science and Technology, CEBAS-CSIC , 30100 Campus de Espinardo, Murcia Spain.
  • Selma MV; Research Group on Quality, Safety, and Bioactivity of Plant Foods, Laboratory of Food & Health, Department of Food Science and Technology, CEBAS-CSIC , 30100 Campus de Espinardo, Murcia Spain.
  • Raes K; Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University , Ghent, Belgium.
  • Smagghe G; Research Group on Quality, Safety, and Bioactivity of Plant Foods, Laboratory of Food & Health, Department of Food Science and Technology, CEBAS-CSIC , 30100 Campus de Espinardo, Murcia Spain.
  • Possemiers S; Department of Industrial Biological Sciences, Faculty of Bioscience Engineering, Ghent University , Kortrijk, Belgium.
  • Van Camp J; Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University , Ghent, Belgium.
  • Tomas-Barberan FA; ProDigest BVBA , Ghent, Belgium.
J Agric Food Chem ; 65(27): 5480-5493, 2017 Jul 12.
Article en En | MEDLINE | ID: mdl-28616977
A TWIN-SHIME system was used to compare the metabolism of pomegranate polyphenols by the gut microbiota from two individuals with different urolithin metabotypes. Gut microbiota, ellagitannin metabolism, short-chain fatty acids (SCFA), transport of metabolites, and phase II metabolism using Caco-2 cells were explored. The simulation reproduced the in vivo metabolic profiles for each metabotype. The study shows for the first time that microbial composition, metabolism of ellagitannins, and SCFA differ between metabotypes and along the large intestine. The assay also showed that pomegranate phenolics preserved intestinal cell integrity. Pomegranate polyphenols enhanced urolithin and propionate production, as well as Akkermansia and Gordonibacter prevalence with the highest effect in the descending colon. The system provides an insight into the mechanisms of pomegranate polyphenol gut microbiota metabolism and absorption through intestinal cells. The results obtained by the combined SHIME/Caco-2 cell system are consistent with previous human and animal studies and show that although urolithin metabolites are present along the gastrointestinal tract due to enterohepatic circulation, they are predominantly produced in the distal colon region.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Extractos Vegetales / Cumarinas / Lythraceae / Tracto Gastrointestinal / Polifenoles / Microbioma Gastrointestinal Tipo de estudio: Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: J Agric Food Chem Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Extractos Vegetales / Cumarinas / Lythraceae / Tracto Gastrointestinal / Polifenoles / Microbioma Gastrointestinal Tipo de estudio: Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: J Agric Food Chem Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos