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Quorum sensing molecule-farnesol increased the production and biological activities of extracellular polysaccharide from Trametes versicolor.
Wang, Ke-Feng; Sui, Kun-Yan; Guo, Chen; Liu, Chun-Zhao.
Afiliación
  • Wang KF; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
  • Sui KY; School of Material Science and Engineering, Qingdao University, Qingdao 266071, PR China.
  • Guo C; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
  • Liu CZ; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; School of Material Science and Engineering, Qingdao University, Qingdao 266071, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China. Ele
Int J Biol Macromol ; 104(Pt A): 377-383, 2017 Nov.
Article en En | MEDLINE | ID: mdl-28610927
A novel strategy of exposing 2-day-old mycelia cultures to 0.8mM farnesol was developed to stimulate extracellular polysaccharide (EPS) production in Trametes versicolor submerged cultures. Farnesol, a quorum sensing molecule in fungi, could significantly increase EPS production by promoting polysaccharide biosynthesis and regulating mycelial morphology. EPS yield reached a maximum of 2.56g/L that was 2.7-fold greater than that of control cultures. Farnesol made T. versicolor develop into fluffy, loose and multi-hyphae morphology, which facilitated the excretion of intracellular polysaccharide into culture medium. Moreover, EPS from farnesol-induced cultures (EPS-F) with higher carbohydrate and uronic acid contents mainly contained high molecular weight polysaccharide (134kDa, 85%), and comprised glucose, mannose and galactose in a molar ratio of 34.2:2.1:1.0. These physicochemical properties led to stronger antioxidant and antitumor activities of EPS-F. This is the first report that farnesol can significantly improve the production of polysaccharide with higher biological activities. It provides a novel strategy to enhance the production and bioactivity of mushroom polysaccharide using microbial quorum sensing molecules.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espacio Extracelular / Percepción de Quorum / Trametes / Farnesol / Polisacáridos Fúngicos Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 2017 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espacio Extracelular / Percepción de Quorum / Trametes / Farnesol / Polisacáridos Fúngicos Límite: Humans Idioma: En Revista: Int J Biol Macromol Año: 2017 Tipo del documento: Article Pais de publicación: Países Bajos