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CXCL12 and osteopontin from bone marrow-derived mesenchymal stromal cells improve muscle regeneration.
Maeda, Yasushi; Yonemochi, Yasuhiro; Nakajyo, Yuki; Hidaka, Hideaki; Ikeda, Tokunori; Ando, Yukio.
Afiliación
  • Maeda Y; Department of Neurology, National Hospital Organization Kumamoto Saishunso National Hospital, Kumamoto, Japan. yasushim@saisyunsou1.hosp.go.jp.
  • Yonemochi Y; Department of Neurology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
  • Nakajyo Y; Department of Neurology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
  • Hidaka H; Department of Neurology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
  • Ikeda T; Department of Clinical Research Center, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.
  • Ando Y; Department of Neurology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
Sci Rep ; 7(1): 3305, 2017 06 12.
Article en En | MEDLINE | ID: mdl-28607396
Muscle satellite cells are essential for muscle regeneration. However, efficient regeneration does not occur without muscle-resident mesenchymal progenitor cells. We show here that bone marrow-derived mesenchymal stromal cells (Bm-MSCs) also facilitate muscle regeneration in Duchenne muscular dystrophy (DMD) model mice. Bm-MSCs transplanted into peritoneal cavities of DMD model mice with severe muscle degeneration strongly suppressed dystrophic pathology and improved death-related symptoms, which resulted in dramatic lifespan extension. Isolated single myofibers from Bm-MSC-transplanted mice manifested considerably less myofiber splitting compared with myofibers from non-transplanted mice, which indicated that transplantation significantly ameliorated abnormal regeneration. With regard to the number of satellite cells, several cells remained on myofibers from Bm-MSC-transplanted model mice, but satellite cells rarely occurred on myofibers from non-transplanted mice. Also, CXCL12 was crucial for muscle regeneration. CXCL12 facilitated muscle regeneration and paired box protein-7 (PAX7) expression after cardiotoxin-related muscle injury in vivo. The majority of primary muscle satellite cells sorted by integrin-α7 and CD34 expressed CXCR4, a receptor specific for CXCL12. CXCL12 strongly suppressed p-STAT3 expression in these sorted cells in vitro. CXCL12 may therefore influence muscle regeneration through STAT3 signaling in satellite cells. Targeting these proteins in or on muscle satellite cells may improve many degenerative muscle diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración / Células de la Médula Ósea / Músculo Esquelético / Osteopontina / Quimiocina CXCL12 / Células Madre Mesenquimatosas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración / Células de la Médula Ósea / Músculo Esquelético / Osteopontina / Quimiocina CXCL12 / Células Madre Mesenquimatosas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Sci Rep Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido