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The fate and lifespan of human monocyte subsets in steady state and systemic inflammation.
Patel, Amit A; Zhang, Yan; Fullerton, James N; Boelen, Lies; Rongvaux, Anthony; Maini, Alexander A; Bigley, Venetia; Flavell, Richard A; Gilroy, Derek W; Asquith, Becca; Macallan, Derek; Yona, Simon.
Afiliación
  • Patel AA; Division of Medicine, University College London, University of London, London, England, UK.
  • Zhang Y; Institute for Infection and Immunity, St. George's, University of London, London, England, UK.
  • Fullerton JN; Division of Medicine, University College London, University of London, London, England, UK.
  • Boelen L; Theoretical Immunology Group, Faculty of Medicine, Imperial College London, London, England, UK.
  • Rongvaux A; Department of Immunobiology, Yale University, New Haven, CT.
  • Maini AA; Division of Medicine, University College London, University of London, London, England, UK.
  • Bigley V; Newcastle University Medical School, Newcastle University, Newcastle Upon Tyne, England, UK.
  • Flavell RA; Department of Immunobiology, Yale University, New Haven, CT.
  • Gilroy DW; Howard Hughes Medical Institute, Yale University, New Haven, CT.
  • Asquith B; Division of Medicine, University College London, University of London, London, England, UK.
  • Macallan D; Theoretical Immunology Group, Faculty of Medicine, Imperial College London, London, England, UK.
  • Yona S; Institute for Infection and Immunity, St. George's, University of London, London, England, UK.
J Exp Med ; 214(7): 1913-1923, 2017 Jul 03.
Article en En | MEDLINE | ID: mdl-28606987
In humans, the monocyte pool comprises three subsets (classical, intermediate, and nonclassical) that circulate in dynamic equilibrium. The kinetics underlying their generation, differentiation, and disappearance are critical to understanding both steady-state homeostasis and inflammatory responses. Here, using human in vivo deuterium labeling, we demonstrate that classical monocytes emerge first from marrow, after a postmitotic interval of 1.6 d, and circulate for a day. Subsequent labeling of intermediate and nonclassical monocytes is consistent with a model of sequential transition. Intermediate and nonclassical monocytes have longer circulating lifespans (∼4 and ∼7 d, respectively). In a human experimental endotoxemia model, a transient but profound monocytopenia was observed; restoration of circulating monocytes was achieved by the early release of classical monocytes from bone marrow. The sequence of repopulation recapitulated the order of maturation in healthy homeostasis. This developmental relationship between monocyte subsets was verified by fate mapping grafted human classical monocytes into humanized mice, which were able to differentiate sequentially into intermediate and nonclassical cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de la Médula Ósea / Monocitos / Diferenciación Celular / Inflamación Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: J Exp Med Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de la Médula Ósea / Monocitos / Diferenciación Celular / Inflamación Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: J Exp Med Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos