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In vitro effects of dalteparin on thrombin generation in canine plasma.
Gara-Boivin, Carolyn; Del Castillo, Jérôme R E; Dunn, Marilyn E; Bédard, Christian.
Afiliación
  • Gara-Boivin C; Department of Pathology and Microbiology, Faculté de Médecine Vétérinaire, Université de Montréal, St. Hyacinthe, QC, Canada.
  • Del Castillo JRE; GREPAQ - Department of Veterinary Biomedicine, Faculté de Médecine Vétérinaire, Université de Montréal, St. Hyacinthe, QC, Canada.
  • Dunn ME; Department of Clinical Sciences, Faculté de Médecine Vétérinaire, Université de Montréal, St. Hyacinthe, QC, Canada.
  • Bédard C; Department of Pathology and Microbiology, Faculté de Médecine Vétérinaire, Université de Montréal, St. Hyacinthe, QC, Canada.
Vet Clin Pathol ; 46(3): 442-450, 2017 Sep.
Article en En | MEDLINE | ID: mdl-28605123
BACKGROUND: The calibrated automated thrombogram (CAT) is a functional thrombin generation (TG) assay that may provide a new approach for monitoring anticoagulant therapy in dogs. The effects of dalteparin on TG variables in dogs are unknown. OBJECTIVES: Objectives were to establish normal TG variable ranges in dogs and measure the in vitro TG variables in canine pooled platelet-poor plasma (PPP) spiked with different dalteparin concentrations. METHODS: In the first experiment, plasma samples from 25 adult healthy Beagle dogs and 11 client-owned healthy dogs of multiple breeds was measured individually for obtaining normal TG values. In the second experiment, separate pools of the remaining PPP from 24 of the 25 previous adult Beagles and from 45 different client-owned dogs were spiked with dalteparin at 9 concentrations with increasing anti-factor Xa (anti-FXa) activity. Activated partial thromboplastin time, tissue factor-induced TG, and anti-FXa activity were measured for each concentration. Concentration-response relationships were determined with ADAPT v.5, using various nonlinear regression models for stimulatory or inhibitory effects. RESULTS: Thrombin generation ranges of client-owned dogs and Beagles were equivalent only for time-to-peak (P < .05). In vitro dalteparin resulted in a concentration-dependent decrease in endogenous thrombin potential (ETP) in pooled PPP. The estimated dalteparin concentration that produced half the maximal inhibition of baseline ETP (IC50 ) was 0.289 U/mL. Thrombin generation and anti-FXa activity were more sensitive than APTT to detect the effects of dalteparin. CONCLUSIONS: The CAT assay can measure the effects of dalteparin in canine plasma, resulting in significant dose-dependent decreases in ETP, prompting further in vivo investigation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombina / Dalteparina / Anticoagulantes Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Vet Clin Pathol Asunto de la revista: MEDICINA VETERINARIA / PATOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombina / Dalteparina / Anticoagulantes Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Vet Clin Pathol Asunto de la revista: MEDICINA VETERINARIA / PATOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos