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GmTIR1/GmAFB3-based auxin perception regulated by miR393 modulates soybean nodulation.
Cai, Zhaoming; Wang, Youning; Zhu, Lin; Tian, Yinping; Chen, Liang; Sun, Zhengxi; Ullah, Ihteram; Li, Xia.
Afiliación
  • Cai Z; State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Wang Y; College of Life Science and Technology, Yangtze Normal University, Chongqing, 408100, China.
  • Zhu L; State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Tian Y; State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
  • Chen L; Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong Road, Shijiazhuang, Hebei, 050021, China.
  • Sun Z; Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong Road, Shijiazhuang, Hebei, 050021, China.
  • Ullah I; Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong Road, Shijiazhuang, Hebei, 050021, China.
  • Li X; University of Chinese Academy of Sciences, Beijing, 100049, China.
New Phytol ; 215(2): 672-686, 2017 Jul.
Article en En | MEDLINE | ID: mdl-28598036
Auxins play important roles in the nodulation of legumes. However, the mechanism by which auxin signaling regulates root nodulation is largely unknown. In particular, the role of auxin receptors and their regulation in determinate nodule development remains elusive. We checked the expression pattern of the auxin receptor GmTIR1/GmAFB3 genes in soybean. We analyzed the functions of GmTIR1/AFB3 in the regulation of rhizobial infection and nodule number, and also tested the functions of miR393 during nodulation and its relationship with GmTIR1/AFB3. The results showed that GmTIR1 and GmAFB3 genes exhibit diverse expression patterns during nodulation and overexpression of GmTIR1 genes significantly increased inflection foci and eventual nodule number. GmTIR1/AFB3 genes were post-transcriptionally cleaved by miR393 family and knock-down of the miR393 family members significantly increased rhizobial infection and the nodule number. Overexpression of the mutated form of GmTIR1C at the miR393 cleavage site that is resistant to miR393 cleavage led to a further increase in the number of infection foci and nodules, suggesting that miR393s modulate nodulation by directly targeting GmTIR1C. This study demonstrated that GmTIR1- and GmAFB3-mediated auxin signaling, that is spatio-temporally regulated by miR393, plays a crucial role in determinate nodule development in soybean.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Glycine max / Nodulación de la Raíz de la Planta / Ácidos Indolacéticos Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Glycine max / Nodulación de la Raíz de la Planta / Ácidos Indolacéticos Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido