Your browser doesn't support javascript.
loading
Glycan profiling using formalin-fixed, paraffin-embedded tissues: Hippeastrum hybrid lectin is a sensitive biomarker for squamous cell carcinoma of the uterine cervix.
Tozawa-Ono, Akiko; Kubota, Manabu; Honma, Chika; Nakagawa, Yuko; Yokomichi, Noriyuki; Yoshioka, Norihito; Tsuda, Chiharu; Ohara, Tatsuru; Koizumi, Hirotaka; Suzuki, Nao.
Afiliación
  • Tozawa-Ono A; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Miyamae, Kawasaki, Japan.
  • Kubota M; Department of Pathology, St. Marianna University School of Medicine, Miyamae, Kawasaki, Japan.
  • Honma C; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Miyamae, Kawasaki, Japan.
  • Nakagawa Y; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Miyamae, Kawasaki, Japan.
  • Yokomichi N; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Miyamae, Kawasaki, Japan.
  • Yoshioka N; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Miyamae, Kawasaki, Japan.
  • Tsuda C; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Miyamae, Kawasaki, Japan.
  • Ohara T; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Miyamae, Kawasaki, Japan.
  • Koizumi H; Department of Pathology, St. Marianna University School of Medicine, Miyamae, Kawasaki, Japan.
  • Suzuki N; Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Miyamae, Kawasaki, Japan.
J Obstet Gynaecol Res ; 43(8): 1326-1334, 2017 Aug.
Article en En | MEDLINE | ID: mdl-28585749
AIM: Glycosylation of proteins is altered in cancer cells and distinctive glycan structures are associated with specific cancers, but little is known about the complete glycan profile of particular tumors. In this study, glycomic analysis of squamous cell carcinoma (SCC) of the uterine cervix was performed to search for useful markers. METHODS: A lectin microarray containing 45 lectins with different binding preferences that covered N- and O-linked glycans was coupled with evanescent field-activated fluorescent detection for glycomic analysis of SCC and normal squamous epithelium (NSE) of the cervix. Formalin-fixed, paraffin-embedded tissue specimens were obtained from 16 patients with uterine cervical cancer. Sections that included both tumor and non-tumor tissues were examined to detect alterations of glycans based on the lectin-binding pattern. RESULTS: Hippeastrum hybrid lectin was found to be a sensitive marker for distinguishing SCC of the cervix from NSE. It was the best lectin for discriminating SCC from other tissues according to receiver-operator curve analysis, as it showed a high sensitivity (81.8%), a high specificity (70.1%), and a large area under the curve (0.8182). Histochemistry confirmed specific cytoplasmic staining of SCC cells by Hippeastrum hybrid lectin, while there was little staining of cervical intraepithelial neoplasia and no staining of NSE. CONCLUSION: The present lectin microarray technique could be applied for tissue-based glycomic analysis of various tumors and for discovery of glycan-related biomarkers.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Carcinoma de Células Escamosas / Neoplasias del Cuello Uterino / Lectinas de Plantas / Amaryllidaceae Tipo de estudio: Diagnostic_studies / Evaluation_studies / Prognostic_studies Límite: Adult / Female / Humans Idioma: En Revista: J Obstet Gynaecol Res Asunto de la revista: GINECOLOGIA / OBSTETRICIA Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Australia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Carcinoma de Células Escamosas / Neoplasias del Cuello Uterino / Lectinas de Plantas / Amaryllidaceae Tipo de estudio: Diagnostic_studies / Evaluation_studies / Prognostic_studies Límite: Adult / Female / Humans Idioma: En Revista: J Obstet Gynaecol Res Asunto de la revista: GINECOLOGIA / OBSTETRICIA Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Australia