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Lipid/alginate nanoparticle-loaded in situ gelling system tailored for dexamethasone nasal delivery.
Dukovski, Bisera Jurisic; Plantic, Ivana; Cuncic, Ivan; Krtalic, Iva; Juretic, Marina; Pepic, Ivan; Lovric, Jasmina; Hafner, Anita.
Afiliación
  • Dukovski BJ; University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, Zagreb, Croatia.
  • Plantic I; University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, Zagreb, Croatia.
  • Cuncic I; University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, Zagreb, Croatia.
  • Krtalic I; R&D, PLIVA Croatia Ltd, TEVA Group Member, Zagreb, Croatia.
  • Juretic M; University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, Zagreb, Croatia.
  • Pepic I; University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, Zagreb, Croatia.
  • Lovric J; University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, Zagreb, Croatia.
  • Hafner A; University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Technology, Zagreb, Croatia. Electronic address: ahafner@pharma.hr.
Int J Pharm ; 533(2): 480-487, 2017 Nov 30.
Article en En | MEDLINE | ID: mdl-28577969
In this study, we suggest the development of nanoparticle loaded in situ gelling system suitable for corticosteroid nasal delivery. We propose lipid/alginate nanoparticles (size 252.3±2.4nm, polydispersity index 0.241, zeta-potential -31.7±1.0mV, dexamethasone (Dex) content 255±7µgml-1) dispersed in pectin solution (5mgml-1) that undergoes a sol-gel phase transition triggered by Ca2+ present in nasal mucosa. The viscoelasticity of gel obtained by mixing nanoparticle suspension in pectin continuous phase with simulated nasal fluid (1:1V/V) is characterised by a log-linear shear thinning viscosity behaviour. Observed viscosity corresponds to the range of viscosities of nasal mucus at physiological as well as under disease conditions. Nanoparticle-loaded gel was biocompatible with the selected epithelial cell model and, in comparison to dexamethasone solution, provided reduction in Dex release (t50% 2.1h and 0.6h, respectively) and moderated transepithelial permeation in vitro (Papp 7.88±0.15 and 9.73±0.57×10-6cms-1, respectively). In conclusion, this study showed the potential of the proposed system to provide local therapeutic effect upon administration of a lower corticosteroid dose and minimize the possibility for adverse effects as it can be easily sprayed as solution and delivered beyond nasal valve, ensure prolonged contact time with nasal mucosa upon gelation, and moderate corticosteroid release and permeation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dexametasona / Sistemas de Liberación de Medicamentos / Alginatos / Nanopartículas / Glucocorticoides / Lípidos Límite: Humans Idioma: En Revista: Int J Pharm Año: 2017 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dexametasona / Sistemas de Liberación de Medicamentos / Alginatos / Nanopartículas / Glucocorticoides / Lípidos Límite: Humans Idioma: En Revista: Int J Pharm Año: 2017 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Países Bajos