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The Centrosome Is a Selective Condensate that Nucleates Microtubules by Concentrating Tubulin.
Woodruff, Jeffrey B; Ferreira Gomes, Beatriz; Widlund, Per O; Mahamid, Julia; Honigmann, Alf; Hyman, Anthony A.
Afiliación
  • Woodruff JB; Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany. Electronic address: woodruff@mpi-cbg.de.
  • Ferreira Gomes B; Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
  • Widlund PO; Department of Chemistry and Molecular Biology, University of Gothenburg, Medicinaregatan 9 c, 40530 Gothenburg, Sweden.
  • Mahamid J; Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.
  • Honigmann A; Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
  • Hyman AA; Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany. Electronic address: hyman@mpi-cbg.de.
Cell ; 169(6): 1066-1077.e10, 2017 Jun 01.
Article en En | MEDLINE | ID: mdl-28575670
Centrosomes are non-membrane-bound compartments that nucleate microtubule arrays. They consist of nanometer-scale centrioles surrounded by a micron-scale, dynamic assembly of protein called the pericentriolar material (PCM). To study how PCM forms a spherical compartment that nucleates microtubules, we reconstituted PCM-dependent microtubule nucleation in vitro using recombinant C. elegans proteins. We found that macromolecular crowding drives assembly of the key PCM scaffold protein SPD-5 into spherical condensates that morphologically and dynamically resemble in vivo PCM. These SPD-5 condensates recruited the microtubule polymerase ZYG-9 (XMAP215 homolog) and the microtubule-stabilizing protein TPXL-1 (TPX2 homolog). Together, these three proteins concentrated tubulin ∼4-fold over background, which was sufficient to reconstitute nucleation of microtubule asters in vitro. Our results suggest that in vivo PCM is a selective phase that organizes microtubule arrays through localized concentration of tubulin by microtubule effector proteins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tubulina (Proteína) / Caenorhabditis elegans / Centrosoma / Proteínas de Ciclo Celular / Proteínas de Caenorhabditis elegans / Microtúbulos Límite: Animals Idioma: En Revista: Cell Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tubulina (Proteína) / Caenorhabditis elegans / Centrosoma / Proteínas de Ciclo Celular / Proteínas de Caenorhabditis elegans / Microtúbulos Límite: Animals Idioma: En Revista: Cell Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos