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Intradiencephalon injection of histamine inhibited the recovery of locomotor function of spinal cord injured zebrafish.
Huang, Shu-Bing; Zhao, Hou-De; Wang, Lin-Fang; Sun, Meng-Fei; Zhu, Ying-Li; Wu, Yi-Bo; Xu, Yi-Da; Peng, Shi-Xiao; Cui, Chun; Shen, Yan-Qin.
Afiliación
  • Huang SB; Neuroscience Center, Jiangnan University Medical School, Wuxi, China.
  • Zhao HD; Neuroscience Center, Jiangnan University Medical School, Wuxi, China.
  • Wang LF; Neuroscience Center, Jiangnan University Medical School, Wuxi, China.
  • Sun MF; Neuroscience Center, Jiangnan University Medical School, Wuxi, China.
  • Zhu YL; Neuroscience Center, Jiangnan University Medical School, Wuxi, China.
  • Wu YB; Neuroscience Center, Jiangnan University Medical School, Wuxi, China; Affiliated Hospital of Jiangnan University, Human Reproductive and Genetic Center, Wuxi, China.
  • Xu YD; Neuroscience Center, Jiangnan University Medical School, Wuxi, China.
  • Peng SX; Neuroscience Center, Jiangnan University Medical School, Wuxi, China.
  • Cui C; Neuroscience Center, Jiangnan University Medical School, Wuxi, China. Electronic address: cuichun@jiangnan.edu.cn.
  • Shen YQ; Neuroscience Center, Jiangnan University Medical School, Wuxi, China. Electronic address: shenyanqin@jiangnan.edu.cn.
Biochem Biophys Res Commun ; 489(3): 275-280, 2017 07 29.
Article en En | MEDLINE | ID: mdl-28559136
Human spinal cord injury (SCI) usually causes irreversible disability beneath the injured site due to poor neural regeneration. On the contrary, zebrafish show significant regenerative ability after SCI, thus is usually worked as an animal model for studying neuroregeneration. Most of the previous SCI studies focused on the local site of SCI, the supraspinal-derived signals were rarely mentioned. Here we showed that intradiencephalon injection of histamine (HA) inhibited the locomotor recovery in adult zebrafish post-SCI. Immunofluorescence results showed that intradiencephalon HA administration increased the activated microglia 3 days post injury (dpi), promoted the proliferation of radial glial cells at 7 dpi and affected the morphology of radial glial cells at 11 dpi. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) results showed that intradiencephalon HA administration also reduced the expression of neurotrophic factors including brain-derived neurotrophic factor (BDNF) and insulin-like growth factor1 (IGF-1) at the lesion site, however, had no effect on the expression of pro-inflammatory factors such as TNF-alpha and IL-1 beta. Hence, our data suggested that exogenous intradiencephalon HA retarded locomotor recovery in spinal cord injured zebrafish via modulating the repair microenvironment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos de la Médula Espinal / Pez Cebra / Histamina / Locomoción Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos de la Médula Espinal / Pez Cebra / Histamina / Locomoción Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos