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Use of protocatechuic acid as the sole antioxidant in the base medium for in vitro culture of ovine isolated secondary follicles.
Menezes, V G; Santos, Jms; Macedo, Tjs; Lins, Tlbg; Barberino, R S; Gouveia, B B; Bezerra, Més; Cavalcante, Ayp; Queiroz, Maa; Palheta, R C; Matos, Mht.
Afiliación
  • Menezes VG; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil.
  • Santos J; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil.
  • Macedo T; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil.
  • Lins T; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil.
  • Barberino RS; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil.
  • Gouveia BB; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil.
  • Bezerra M; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil.
  • Cavalcante A; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil.
  • Queiroz M; Laboratory of Bromatology and Animal Nutrition, Federal University of São Francisco Valley, Petrolina, PE, Brazil.
  • Palheta RC; Laboratory of Pharmacology, Federal University of São Francisco Valley, Petrolina, PE, Brazil.
  • Matos M; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil.
Reprod Domest Anim ; 52(5): 890-898, 2017 Oct.
Article en En | MEDLINE | ID: mdl-28556248
This study evaluated the effect of the protocatechuic acid (PCA) as the sole antioxidant in the base medium for in vitro culture of ovine secondary follicles. Secondary follicles (200-230 µm) were isolated and cultured in α-minimal essential medium supplemented with BSA, insulin, glutamine and hypoxanthine (α-MEM: antioxidant-free medium) or α-MEM also added by transferrin, selenium and ascorbic acid (α-MEM+: with antioxidant) or α-MEM added by PCA (56.25; 112.5; 225; 450; or 900 µg/ml). Moreover, after culture, oocytes were matured and the chromatin configuration and DNA fragmentation were evaluated. After 12 days, the treatment containing 56.25 µg/ml PCA showed higher percentage of normal follicles than control medium or the other treatments (p < .05), except for 900 µg/ml PCA (p > .05). The antrum formation was significantly higher in treatments containing 56.25, 112.5 or 900 µg/ml PCA, compared to the α-MEM and similar (p > .05) to the other treatments. The rates of fully grown oocytes (≥110 µm) were similar (p > .05) among all treatments containing PCA and α-MEM+, and those were superior (p < .05) than α-MEM, except for 450 µg/ml PCA (p > .05). GSH levels and mitochondrial activity were higher (p < .05) in α-MEM+ than in α-MEM and similar (p > .05) to all PCA treatments. The rates of meiotic resumption and DNA fragmentation were similar (p > .05) among α-MEM+ and 56.25 µg/ml PCA. In conclusion, PCA at 56.25 µg/ml as the sole antioxidant added to the medium for ovine isolated secondary follicle culture maintains follicular survival, GSH and active mitochondria levels, meiotic developmental competence and DNA integrity of cultured oocytes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oveja Doméstica / Folículo Ovárico / Hidroxibenzoatos / Antioxidantes Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oveja Doméstica / Folículo Ovárico / Hidroxibenzoatos / Antioxidantes Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania