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PEO-PPO Diblock Copolymers Protect Myoblasts from Hypo-Osmotic Stress In Vitro Dependent on Copolymer Size, Composition, and Architecture.
Kim, Mihee; Haman, Karen J; Houang, Evelyne M; Zhang, Wenjia; Yannopoulos, Demetris; Metzger, Joseph M; Bates, Frank S; Hackel, Benjamin J.
Afiliación
  • Kim M; Department of Chemical Engineering and Materials Science, ‡Department of Integrative Biology and Physiology, and §Department of Medicine, Cardiovascular Division, University of Minnesota , Minneapolis, Minnesota 55455, United States.
  • Haman KJ; Department of Chemical Engineering and Materials Science, ‡Department of Integrative Biology and Physiology, and §Department of Medicine, Cardiovascular Division, University of Minnesota , Minneapolis, Minnesota 55455, United States.
  • Houang EM; Department of Chemical Engineering and Materials Science, ‡Department of Integrative Biology and Physiology, and §Department of Medicine, Cardiovascular Division, University of Minnesota , Minneapolis, Minnesota 55455, United States.
  • Zhang W; Department of Chemical Engineering and Materials Science, ‡Department of Integrative Biology and Physiology, and §Department of Medicine, Cardiovascular Division, University of Minnesota , Minneapolis, Minnesota 55455, United States.
  • Yannopoulos D; Department of Chemical Engineering and Materials Science, ‡Department of Integrative Biology and Physiology, and §Department of Medicine, Cardiovascular Division, University of Minnesota , Minneapolis, Minnesota 55455, United States.
  • Metzger JM; Department of Chemical Engineering and Materials Science, ‡Department of Integrative Biology and Physiology, and §Department of Medicine, Cardiovascular Division, University of Minnesota , Minneapolis, Minnesota 55455, United States.
  • Bates FS; Department of Chemical Engineering and Materials Science, ‡Department of Integrative Biology and Physiology, and §Department of Medicine, Cardiovascular Division, University of Minnesota , Minneapolis, Minnesota 55455, United States.
  • Hackel BJ; Department of Chemical Engineering and Materials Science, ‡Department of Integrative Biology and Physiology, and §Department of Medicine, Cardiovascular Division, University of Minnesota , Minneapolis, Minnesota 55455, United States.
Biomacromolecules ; 18(7): 2090-2101, 2017 Jul 10.
Article en En | MEDLINE | ID: mdl-28535058
Poloxamer 188, a triblock copolymer of poly(ethylene oxide) (PEO) and poly(propylene oxide) (PPO), protects cellular membranes from various stresses. Though numerous block copolymer variants exist, evaluation of alternative architecture, composition, and size has been minimal. Herein, cultured murine myoblasts are exposed to the stresses of hypotonic shock and isotonic recovery, and membrane integrity was evaluated by quantifying release of lactate dehydrogenase. Comparative evaluation of a systematic set of PEO-PPO diblock and PEO-PPO-PEO triblock copolymers demonstrates that the diblock architecture can be protective in vitro. Short PPO blocks hinder protection with >9 PPO units needed for protection at 150 µM and >16 units needed at 14 µM. Addition of a tert-butyl end group enhances protection at reduced concentration. When the end group and PPO length are fixed, increasing the PEO length improves protection. This systematic evaluation establishes a new in vitro screening tool for evaluating membrane-sealing amphiphiles and provides mechanistic insight to guide future copolymer design for membrane stabilization in vivo.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Presión Osmótica / Polietilenglicoles / Polipropilenos / Mioblastos Límite: Animals Idioma: En Revista: Biomacromolecules Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Presión Osmótica / Polietilenglicoles / Polipropilenos / Mioblastos Límite: Animals Idioma: En Revista: Biomacromolecules Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos