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The superoxide dismutase mimetic tempol does not alleviate glucocorticoid-mediated rarefaction of rat skeletal muscle capillaries.
Mandel, Erin R; Dunford, Emily C; Abdifarkosh, Ghoncheh; Turnbull, Patrick C; Perry, Christopher G R; Riddell, Michael C; Haas, Tara L.
Afiliación
  • Mandel ER; School of Kinesiology and Health Science and the Muscle Health Research Centre, York University, Toronto, Ontario, Canada.
  • Dunford EC; School of Kinesiology and Health Science and the Muscle Health Research Centre, York University, Toronto, Ontario, Canada.
  • Abdifarkosh G; School of Kinesiology and Health Science and the Muscle Health Research Centre, York University, Toronto, Ontario, Canada.
  • Turnbull PC; School of Kinesiology and Health Science and the Muscle Health Research Centre, York University, Toronto, Ontario, Canada.
  • Perry CGR; School of Kinesiology and Health Science and the Muscle Health Research Centre, York University, Toronto, Ontario, Canada.
  • Riddell MC; School of Kinesiology and Health Science and the Muscle Health Research Centre, York University, Toronto, Ontario, Canada.
  • Haas TL; School of Kinesiology and Health Science and the Muscle Health Research Centre, York University, Toronto, Ontario, Canada.
Physiol Rep ; 5(10): e13243, 2017 May.
Article en En | MEDLINE | ID: mdl-28533261
Sustained elevations in circulating glucocorticoids elicit reductions in skeletal muscle microvascular content, but little is known of the underlying mechanisms. We hypothesized that glucocorticoid-induced oxidative stress contributes to this phenomenon. In rats that were implanted with corticosterone (CORT) or control pellets, CORT caused a significant decrease in muscle glutathione levels and a corresponding increase in protein carbonylation, an irreversible oxidative modification of proteins. Decreased endothelial nitric oxide synthase and increased endothelin-1 mRNA levels were detected after 9 days of CORT, and blood flow to glycolytic muscles was diminished. Control and CORT rats were treated concurrently with drinking water containing the superoxide dismutase mimetic tempol (172 mg/L) or the α-1 adrenergic receptor antagonist prazosin (50 mg/L) for 6 or 16 days. Both tempol and prazosin alleviated skeletal muscle protein carbonylation. Tempol failed to prevent CORT-mediated capillary rarefaction and was ineffective in restoring skeletal muscle blood flow. In contrast, prazosin blocked capillary rarefaction and restored skeletal muscle blood flow to control levels. The failure of tempol to prevent CORT-induced skeletal muscle microvascular rarefaction does not support a dominant role of superoxide-induced oxidative stress in this process. Although a decrease in protein carbonylation was observed with prazosin treatment, our data suggest that the maintenance of skeletal muscle microvascular content is related more closely with counteracting the CORT-mediated influence on skeletal muscle vascular tone.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Superóxido Dismutasa / Corticosterona / Estrés Oxidativo / Músculo Esquelético / Óxidos N-Cíclicos Límite: Animals Idioma: En Revista: Physiol Rep Año: 2017 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Superóxido Dismutasa / Corticosterona / Estrés Oxidativo / Músculo Esquelético / Óxidos N-Cíclicos Límite: Animals Idioma: En Revista: Physiol Rep Año: 2017 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos