Zinc oxide nanoparticle energy band gap reduction triggers the oxidative stress resulting into autophagy-mediated apoptotic cell death.

Arakha, Manoranjan; Roy, Jyoti; Nayak, Parth Sarthi; Mallick, Bibekanand; Jha, Suman

Arakha, Manoranjan; Roy, Jyoti; Nayak, Parth Sarthi; Mallick, Bibekanand; Jha, Suman.

Afiliación

Arakha M; Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.
Roy J; Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.
Nayak PS; Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.
Mallick B; Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.
Jha S; Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India. Electronic address: jhas@nitrkl.ac.in.

Free Radic Biol Med ; 110: 42-53, 2017 09.

Article en En | MEDLINE | ID: mdl-28528796

RESUMEN

The physico-chemical properties of nanoparticle (NP), such as particle size, surface defects, crystallinity and accessible surface, affect NP photocatalytic activity that in turn defines the NP cytotoxic propensity. Since zinc oxide nanoparticle (ZnONP) energy band gap falls in a range of a semiconductor, the particle possesses photocatalytic activity. Hence, the study correlates energy band gap with cytotoxic propensity of ZnONP. To this end, ZnONPs with varying energy band gap are fabricated by varying calcination temperature. Cytotoxic propensity of the fabricated ZnONPs against HT1080 cell indicates that the particle with least energy band gap shows highest cytotoxicity. The data also indicate that the cytotoxicity is triggered primarily through reactive oxygen species (ROS)-mediated pathway. Additionally, the comet assay and Î³H2AX activity assay reveal that decreasing energy band gap of the particle increases DNA damaging propensity. Furthermore, cell cycle analysis indicates that the cell treatment with decreasing energy band gap ZnONP results in significant increase in cell population fraction in subG1 phase. Whereas, acridine orange binding assay and increased expression level of LC3II indicate that the cell tries to recover the stress by scavenging damaged cellular biomolecules and ROS using autophagosomes. Nevertheless, cell with the non-recoverable damages led into apoptotic cell death, as confirmed by Annexin V apoptosis assay, DNA fragmentation assay and 4,6-Diamidino-2-phenylindole dihydrochloride (DAPI) staining.

Asunto(s)

Citotoxinas/farmacología; Fibroblastos/efectos de los fármacos; Regulación Neoplásica de la Expresión Génica; Nanopartículas/toxicidad; Especies Reactivas de Oxígeno/agonistas; Óxido de Zinc/farmacología; Anexina A5/genética; Anexina A5/metabolismo; Apoptosis/efectos de los fármacos; Autofagia/efectos de los fármacos; Ciclo Celular/efectos de los fármacos; Ciclo Celular/genética; Línea Celular Tumoral; Ensayo Cometa; Fragmentación del ADN/efectos de los fármacos; Fibroblastos/metabolismo; Fibroblastos/patología; Histonas/genética; Histonas/metabolismo; Humanos; Proteínas Asociadas a Microtúbulos/genética; Proteínas Asociadas a Microtúbulos/metabolismo; Estrés Oxidativo; Tamaño de la Partícula; Procesos Fotoquímicos; Especies Reactivas de Oxígeno/metabolismo; Transducción de Señal

Palabras clave

Apoptosis; Energy band gap; Nanocytotoxicity; ROS; Zinc oxide nanoparticle

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Óxido de Zinc / Regulación Neoplásica de la Expresión Génica / Especies Reactivas de Oxígeno / Citotoxinas / Nanopartículas / Fibroblastos Límite: Humans Idioma: En Revista: Free Radic Biol Med Asunto de la revista: BIOQUIMICA / MEDICINA Año: 2017 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos

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