Genetic evidence for the existence of a repressor that modulates colicin D expression on plasmid ColD-CA23.
Mol Gen Genet
; 213(1): 36-41, 1988 Jul.
Article
en En
| MEDLINE
| ID: mdl-2851708
The plasmid ColD-CA23, a high copy number plasmid of 5.12 kb, contains genes for colicin D (cda), for immunity colicin D (cdi), and for a lysis function (cdl). These genes are arranged on a contiguous 2.4 kb fragment in the following sequence: cda, cdi, cdl. They are transcribed in two operons, one transcribing cda and cdl from a SOS inducible promoter, the other transcribing cdi in the opposite direction. The expression of cda and cdl is modulated by a repressor, cdr, which is encoded on the same transcript as cda and cdl. In the absence of this repressor, transcription from the SOS inducible colicin D promoter is exceptionally strong and leads to protein contents up to 50% of total cellular proteins. This autoregulative repressor is a new finding in the control mechanisms of expression of colicins. We have also identified the gene product of cdl to be a 10,000 dalton protein.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plásmidos
/
Proteínas Represoras
/
Factores de Transcripción
/
Regulación de la Expresión Génica
/
Colicinas
/
Escherichia coli
/
Genes Bacterianos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Mol Gen Genet
Año:
1988
Tipo del documento:
Article
País de afiliación:
Suiza
Pais de publicación:
Alemania