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Effect of chitosan and coagulation factors on the wound repair phenotype of bioengineered blood clots.
Hoemann, Caroline D; Marchand, Catherine; Rivard, Georges-Etienne; El-Gabalawy, Hani; Poubelle, Patrice E.
Afiliación
  • Hoemann CD; Department of Chemical Engineering, Ecole Polytechnique, Montréal, QC, Canada; Institute of Biomedical Engineering, Ecole Polytechnique, Montréal, QC, Canada. Electronic address: c.hoemann@polymtl.ca.
  • Marchand C; Institute of Biomedical Engineering, Ecole Polytechnique, Montréal, QC, Canada. Electronic address: catherine-2.marchand@polymtl.ca.
  • Rivard GE; Division of Hematology-Oncology, CHU Sainte-Justine, Montréal, QC, Canada, Canada. Electronic address: georges-etienne.rivard.hsj@ssss.gouv.qc.ca.
  • El-Gabalawy H; Rheumatic Diseases Research Laboratory, University of Manitoba, Winnipeg, MB, Canada. Electronic address: Hani.Elgabalawy@med.umanitoba.ca.
  • Poubelle PE; Department of Rheumatology, Laval University, Québec, QC, Canada. Electronic address: Patrice.Poubelle@crchul.ulaval.ca.
Int J Biol Macromol ; 104(Pt B): 1916-1924, 2017 Nov.
Article en En | MEDLINE | ID: mdl-28512053
Controlling the blood clot phenotype in a surgically prepared wound is an evolving concept in scaffold-guided tissue engineering. Here, we investigated the effect of added chitosan (80% or 95% Degree of Deacetylation, DDA) or coagulation factors (recombinant human Factor VIIa, Tissue Factor, thrombin) on inflammatory factors released by blood clots. We tested the hypothesis that 80% DDA chitosan specifically enhances leukotriene B4 (LTB4) production. Human or rabbit whole blood was combined with isotonic chitosan solutions, coagulation factors, or lipopolysaccharide, cultured in vitro at 37°C, and after 4hours the serum was assayed for LTB4 or inflammatory factors. Only 80% DDA chitosan clots produced around 15-fold more LTB4 over other clots including 95% DDA chitosan clots. All serum contained high levels of PDGF-BB and CXCL8. Normal clots produced very low type I cytokines compared to lipopolysaccharide clots, with even lower IL-6 and IL-12 and more CCL3/CCL4 produced by chitosan clots. Coagulation factors had no detectable effect on clot phenotype. Conclusion In blood clots from healthy individuals, 80% DDA chitosan has a unique influence of inducing more LTB4, a potent neutrophil chemoattractant, with similar production of PDGF-BB and CXCL8, and lower type I cytokines, compared to whole blood clots.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombosis / Cicatrización de Heridas / Materiales Biocompatibles / Factores de Coagulación Sanguínea / Quitosano / Andamios del Tejido Límite: Adult / Animals / Humans / Middle aged Idioma: En Revista: Int J Biol Macromol Año: 2017 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombosis / Cicatrización de Heridas / Materiales Biocompatibles / Factores de Coagulación Sanguínea / Quitosano / Andamios del Tejido Límite: Adult / Animals / Humans / Middle aged Idioma: En Revista: Int J Biol Macromol Año: 2017 Tipo del documento: Article Pais de publicación: Países Bajos