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Combined Delivery of a Lipopolysaccharide-Binding Peptide and the Heme Oxygenase-1 Gene Using Deoxycholic Acid-Conjugated Polyethylenimine for the Treatment of Acute Lung Injury.
Kim, Ji Yeon; Piao, Chunxian; Kim, Gyeungyun; Lee, Seonyeong; Lee, Min Sang; Jeong, Ji Hoon; Lee, Minhyung.
Afiliación
  • Kim JY; Department of Bioengineering, College of Engineering, Hanyang University, Seoul, 04763, South Korea.
  • Piao C; Department of Bioengineering, College of Engineering, Hanyang University, Seoul, 04763, South Korea.
  • Kim G; Department of Bioengineering, College of Engineering, Hanyang University, Seoul, 04763, South Korea.
  • Lee S; Department of Bioengineering, College of Engineering, Hanyang University, Seoul, 04763, South Korea.
  • Lee MS; School of Pharmacy, Sungkyunkwan University, Suwon, 16419, South Korea.
  • Jeong JH; School of Pharmacy, Sungkyunkwan University, Suwon, 16419, South Korea.
  • Lee M; Department of Bioengineering, College of Engineering, Hanyang University, Seoul, 04763, South Korea.
Macromol Biosci ; 17(8)2017 08.
Article en En | MEDLINE | ID: mdl-28508430
A ternary complex comprising plasmid DNA, lipopolysaccharide-binding peptide (LBP), and deoxycholic acid-conjugated polyethylenimine (PEI-DA) is prepared for combinational therapy of acute lung injury (ALI). The LBP is designed as an anti-inflammatory peptide based on the lipopolysaccharide (LPS)-binding domain of HMGB-1. In vitro cytokine assays show that LBP reduces levels of proinflammatory cytokines by inhibiting LPS. PEI-DA is synthesized as the gene carrier by conjugation of deoxycholic acid to low-molecular weight polyethylenimine (2 kDa, PEI2k). PEI-DA has higher transfection efficiency than high-molecular weight polyethylenimine (25 kDa, PEI25k). The ternary complex of an HO-1 plasmid (pHO-1), PEI-DA, and LBP is prepared as a combinational system to deliver the therapeutic gene and peptide. The transfection efficiency of the ternary complex is higher than that of the pHO-1/PEI-DA binary complex. The ternary complex also reduces TNF-α secretion in LPS-activated Raw264.7 macrophage cells. Administration of the ternary complex into the lungs of an animal ALI model by intratracheal injection induces HO-1 expression and reduces levels of proinflammatory cytokines more efficiently than the pHO-1/PEI-DA binary complex or LBP alone. In addition, the ternary complex reduces inflammation in the lungs. Therefore, the pHO-1/PEI-DA/LBP ternary complex may be an effective treatment for ALI.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polietileneimina / Proteínas de Fase Aguda / Glicoproteínas de Membrana / Proteínas Portadoras / Técnicas de Transferencia de Gen / Ácido Desoxicólico / Hemo-Oxigenasa 1 / Lesión Pulmonar Aguda / Proteínas de la Membrana Límite: Animals Idioma: En Revista: Macromol Biosci Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polietileneimina / Proteínas de Fase Aguda / Glicoproteínas de Membrana / Proteínas Portadoras / Técnicas de Transferencia de Gen / Ácido Desoxicólico / Hemo-Oxigenasa 1 / Lesión Pulmonar Aguda / Proteínas de la Membrana Límite: Animals Idioma: En Revista: Macromol Biosci Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Alemania