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Anti-hyperalgesic effect of Lippia grata leaf essential oil complexed with ß-cyclodextrin in a chronic musculoskeletal pain animal model: Complemented with a molecular docking and antioxidant screening.
Siqueira-Lima, Pollyana S; Brito, Renan G; Araújo-Filho, Heitor G; Santos, Priscila L; Lucchesi, Angélica; Araújo, Adriano A S; Menezes, Paula P; Scotti, Luciana; Scotti, Marcus T; Menezes, Irwin R A; Coutinho, Henrique D M; Zengin, Gokhan; Aktumsek, Abdurrahman; Antoniolli, Angelo R; Quintans-Júnior, Lucindo J; Quintans, Jullyana S S.
Afiliación
  • Siqueira-Lima PS; Laboratory of Neuroscience and Pharmacological Assays (LANEF), Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil; Graduate Program of Biotechnology (PPGBiotec), The State University of Feira de Santana, Feira de Santana, BA, Brazil.
  • Brito RG; Laboratory of Neuroscience and Pharmacological Assays (LANEF), Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil.
  • Araújo-Filho HG; Laboratory of Neuroscience and Pharmacological Assays (LANEF), Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil.
  • Santos PL; Laboratory of Neuroscience and Pharmacological Assays (LANEF), Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil.
  • Lucchesi A; Graduate Program of Biotechnology (PPGBiotec), The State University of Feira de Santana, Feira de Santana, BA, Brazil.
  • Araújo AAS; Department of Pharmacy (DFA), Federal University of Sergipe, São Cristóvão, SE, Brazil.
  • Menezes PP; Department of Pharmacy (DFA), Federal University of Sergipe, São Cristóvão, SE, Brazil.
  • Scotti L; Graduate Program of Natural Product and Bioactive Synthetics, Federal University of Paraíba, João Pessoa, PB, Brazil.
  • Scotti MT; Graduate Program of Natural Product and Bioactive Synthetics, Federal University of Paraíba, João Pessoa, PB, Brazil.
  • Menezes IRA; Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil.
  • Coutinho HDM; Department of Biological Chemistry, Regional University of Cariri, Crato, Ceará, Brazil.
  • Zengin G; Department of Biology, Science Faculty, Selcuk University, Campus, 42250, Konya, Turkey.
  • Aktumsek A; Department of Biology, Science Faculty, Selcuk University, Campus, 42250, Konya, Turkey.
  • Antoniolli AR; Laboratory of Neuroscience and Pharmacological Assays (LANEF), Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil.
  • Quintans-Júnior LJ; Laboratory of Neuroscience and Pharmacological Assays (LANEF), Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil. Electronic address: lucindojr@gmail.com.
  • Quintans JSS; Laboratory of Neuroscience and Pharmacological Assays (LANEF), Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil. Electronic address: jullyanaquintans@gmail.com.
Biomed Pharmacother ; 91: 739-747, 2017 Jul.
Article en En | MEDLINE | ID: mdl-28499245
BACKGROUND: Due to its unclear pathophysiology, the pharmacological treatment of fibromyalgia is a challenge for researchers. Studies using medicinal plants, such as those from the genus Lippia, complexed with cyclodextrins (CDs) have shown innovative results. OBJECTIVE: The present research intended to evaluate the effect of an inclusion complex containing ß-cyclodextrin (ßCD) inclusion complex with Lippia grata (LG) essential oil in a chronic musculoskeletal pain model, its central activity and its possible interaction with neurotransmitters involved in pain. METHODS: After acid saline-induced chronic muscle pain, male mice were evaluated for primary and secondary hyperalgesia and muscle strength. Moreover, an antagonist assay was performed to assess the possible involvement of the opioidergic, serotonergic and noradrenergic pathways. In addition, Fos protein in the spinal cord was assessed, and a docking study and antioxidant assays were performed. RESULTS: The treatment with LG-ßCD, especially in the dose of 24mg/kg, was able to significantly decrease (p<0.05) the paw withdrawal and muscle threshold. Furthermore, LG-ßCD was shown to affect the opioidergic and serotonergic pathways. There were no significant changes in muscle strength. Fos protein immunofluorescence showed a significant decrease in expression in the dorsal horn of the spinal cord. The main compounds of LG showed through the docking study interaction energies with the alpha-adrenergic and µOpioid receptors. In all antioxidant assays, LG exhibited stronger antioxidant activities than LG-ßCD. CONCLUSION: This study suggested that LG-ßCD could be considered as a valuable source for designing new drugs in the treatment of chronic pain, especially musculoskeletal pain.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aceites Volátiles / Lippia / Beta-Ciclodextrinas / Dolor Musculoesquelético / Dolor Crónico / Simulación del Acoplamiento Molecular / Hiperalgesia / Antioxidantes Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Animals Idioma: En Revista: Biomed Pharmacother Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Francia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aceites Volátiles / Lippia / Beta-Ciclodextrinas / Dolor Musculoesquelético / Dolor Crónico / Simulación del Acoplamiento Molecular / Hiperalgesia / Antioxidantes Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Animals Idioma: En Revista: Biomed Pharmacother Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Francia