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Essential Roles of SATB1 in Specifying T Lymphocyte Subsets.
Kakugawa, Kiyokazu; Kojo, Satoshi; Tanaka, Hirokazu; Seo, Wooseok; Endo, Takaho A; Kitagawa, Yohko; Muroi, Sawako; Tenno, Mari; Yasmin, Nighat; Kohwi, Yoshinori; Sakaguchi, Shimon; Kowhi-Shigematsu, Terumi; Taniuchi, Ichiro.
Afiliación
  • Kakugawa K; Laboratory for Transcriptional Regulation, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
  • Kojo S; Laboratory for Transcriptional Regulation, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
  • Tanaka H; Laboratory for Transcriptional Regulation, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
  • Seo W; Laboratory for Transcriptional Regulation, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
  • Endo TA; Laboratory for Integrative Genomics, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
  • Kitagawa Y; Department of Experimental Immunology, World Premier International Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan.
  • Muroi S; Laboratory for Transcriptional Regulation, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
  • Tenno M; Laboratory for Transcriptional Regulation, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
  • Yasmin N; Laboratory for Transcriptional Regulation, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
  • Kohwi Y; Life Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
  • Sakaguchi S; Laboratory for Integrative Genomics, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.
  • Kowhi-Shigematsu T; Life Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
  • Taniuchi I; Laboratory for Transcriptional Regulation, RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan. Electronic address: ichiro.taniuchi@riken.jp.
Cell Rep ; 19(6): 1176-1188, 2017 05 09.
Article en En | MEDLINE | ID: mdl-28494867
T cell receptor (TCR) signaling by MHC class I and II induces thymocytes to acquire cytotoxic and helper fates via the induction of Runx3 and ThPOK transcription factors, respectively. The mechanisms by which TCR signaling is translated into transcriptional programs for each cell fate remain elusive. Here, we show that, in post-selection thymocytes, a genome organizer, SATB1, activates genes for lineage-specifying factors, including ThPOK, Runx3, CD4, CD8, and Treg factor Foxp3, via regulating enhancers in these genes in a locus-specific manner. Indeed, SATB1-deficient thymocytes are partially re-directed into inappropriate T lineages after both MHC class I- and II-mediated selection, and they fail to generate NKT and Treg subsets. Despite its essential role in activating enhancers for the gene encoding ThPOK in TCR-signaled thymocytes, SATB1 becomes dispensable for maintaining ThPOK in CD4+ T cells. Collectively, our findings demonstrate that SATB1 shapes the primary T cell pool by directing lineage-specific transcriptional programs in the thymus.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Subgrupos de Linfocitos T / Proteínas de Unión a la Región de Fijación a la Matriz / Linfopoyesis Límite: Animals Idioma: En Revista: Cell Rep Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Subgrupos de Linfocitos T / Proteínas de Unión a la Región de Fijación a la Matriz / Linfopoyesis Límite: Animals Idioma: En Revista: Cell Rep Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos