Avenacosides: Metabolism, and potential use as exposure biomarkers of oat intake.
Mol Nutr Food Res
; 61(7)2017 07.
Article
en En
| MEDLINE
| ID: mdl-28493602
SCOPE: Exposure biomarkers used for objective estimation of whole-grain (WG) intake are essential for epidemiologic studies of WG consumption, however, up to now, no exposure biomarkers were developed for WG oat intake. This study investigates the potential of oat unique components, Avenacoside-B (AVE-B) and -A (AVE-A), as exposure biomarkers of oat intake. METHODS AND RESULTS: An in vivo study performed in mice and an in vitro batch fecal fermentation study were used to investigate the potential metabolic routes of AVE-B and -A. Twelve healthy volunteers were recruited in the human urinary pharmacokinetic study, each participant received a single dose of oat bran as breakfast, 48 h urine samples were collected at baseline and after treatment period, and AVE-B and -A were quantified by LC-MS/MS. Deglycosylation metabolic route was identified as the major metabolic path for AVE-B and -A. Urinary AVE-B and -A concentrations increased rapidly after oat ingestion, reached their maximum excretion rates (ERmax ) fairly simultaneously within 5 h, then decreased gradually. And the mean eliminate half-lives (T1/2 ) for AVE-B and -A were determined as 6.22 and 4.55 h, respectively. CONCLUSION: Oat AVE-B and -A have great potential to be used as specific exposure biomarkers to reflect oat intake.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saponinas
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Avena
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Microbioma Gastrointestinal
Límite:
Adult
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Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
Mol Nutr Food Res
Asunto de la revista:
CIENCIAS DA NUTRICAO
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Alemania