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Dynamic Rewiring of Promoter-Anchored Chromatin Loops during Adipocyte Differentiation.
Siersbæk, Rasmus; Madsen, Jesper Grud Skat; Javierre, Biola Maria; Nielsen, Ronni; Bagge, Emilie Kristine; Cairns, Jonathan; Wingett, Steven William; Traynor, Sofie; Spivakov, Mikhail; Fraser, Peter; Mandrup, Susanne.
Afiliación
  • Siersbæk R; University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
  • Madsen JGS; University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
  • Javierre BM; Nuclear Dynamics Programme, Babraham Institute, Cambridge CB22 3AT, UK.
  • Nielsen R; University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
  • Bagge EK; University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
  • Cairns J; Nuclear Dynamics Programme, Babraham Institute, Cambridge CB22 3AT, UK.
  • Wingett SW; Nuclear Dynamics Programme, Babraham Institute, Cambridge CB22 3AT, UK; Bioinformatics Group, Babraham Institute, Cambridge CB22 3AT, UK.
  • Traynor S; University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
  • Spivakov M; Nuclear Dynamics Programme, Babraham Institute, Cambridge CB22 3AT, UK.
  • Fraser P; Nuclear Dynamics Programme, Babraham Institute, Cambridge CB22 3AT, UK; Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA. Electronic address: peter.fraser@babraham.ac.uk.
  • Mandrup S; University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark. Electronic address: s.mandrup@bmb.sdu.dk.
Mol Cell ; 66(3): 420-435.e5, 2017 May 04.
Article en En | MEDLINE | ID: mdl-28475875
Interactions between transcriptional promoters and their distal regulatory elements play an important role in transcriptional regulation; however, the extent to which these interactions are subject to rapid modulations in response to signals is unknown. Here, we use promoter capture Hi-C to demonstrate a rapid reorganization of promoter-anchored chromatin loops within 4 hr after inducing differentiation of 3T3-L1 preadipocytes. The establishment of new promoter-enhancer loops is tightly coupled to activation of poised (histone H3 lysine 4 mono- and dimethylated) enhancers, as evidenced by the acquisition of histone H3 lysine 27 acetylation and the binding of MED1, SMC1, and P300 proteins to these regions, as well as to activation of target genes. Intriguingly, formation of loops connecting activated enhancers and promoters is also associated with extensive recruitment of corepressors such as NCoR and HDACs, indicating that this class of coregulators may play a previously unrecognized role during enhancer activation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatina / Regiones Promotoras Genéticas / Adipocitos / Ensamble y Desensamble de Cromatina / Adipogénesis Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Mol Cell Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatina / Regiones Promotoras Genéticas / Adipocitos / Ensamble y Desensamble de Cromatina / Adipogénesis Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Mol Cell Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Estados Unidos