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Characterization of Microsatellites in Pseudogymnoascus destructans for White-nose Syndrome Genetic Analysis.
Drees, Kevin P; Parise, Katy L; Rivas, Stephanie M; Felton, Lindsey L; Puechmaille, Sébastien J; Keim, Paul; Foster, Jeffrey T.
Afiliación
  • Drees KP; 1 The Pathogen & Microbiome Institute, Northern Arizona University, PO Box 4073, Flagstaff, Arizona 86011, USA.
  • Parise KL; 3 Current address: Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, 46 College Rd., Durham, New Hampshire 03824, USA.
  • Rivas SM; 1 The Pathogen & Microbiome Institute, Northern Arizona University, PO Box 4073, Flagstaff, Arizona 86011, USA.
  • Felton LL; 3 Current address: Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, 46 College Rd., Durham, New Hampshire 03824, USA.
  • Puechmaille SJ; 1 The Pathogen & Microbiome Institute, Northern Arizona University, PO Box 4073, Flagstaff, Arizona 86011, USA.
  • Keim P; 1 The Pathogen & Microbiome Institute, Northern Arizona University, PO Box 4073, Flagstaff, Arizona 86011, USA.
  • Foster JT; 2 Institute of Zoology, University of Greifswald, Friedrich-Ludwig-Jahn-Str. 15a, D-17489 Greifswald, Germany.
J Wildl Dis ; 53(4): 869-874, 2017 10.
Article en En | MEDLINE | ID: mdl-28475452
Despite only emerging in the past decade, white-nose syndrome has become among the most devastating wildlife diseases known. The pathogenic fungus Pseudogymnoascus destructans infects hibernating bats and typically leads to high rates of mortality at hibernacula during winter in North America. We developed a set of genetic markers to better differentiate P. destructans isolates. We designed and successfully characterized these 23 microsatellite markers of P. destructans for use in disease ecology and epidemiology research. We validated these loci with DNA extracted from a collection of P. destructans isolates from the US and Canada, as well as from Europe (the likely introduction source based on currently available data). Genetic diversity calculated for each locus and for the multilocus panel as a whole indicates sufficient allelic diversity to differentiate among and between samples from both Europe and North America. Indices of genetic diversity indicate a loss of allelic diversity that is consistent with the recent introduction and rapid spread of an emerging pathogen.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ascomicetos / Quirópteros / Micosis Límite: Animals País/Región como asunto: America do norte Idioma: En Revista: J Wildl Dis Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ascomicetos / Quirópteros / Micosis Límite: Animals País/Región como asunto: America do norte Idioma: En Revista: J Wildl Dis Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos