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Supercritical CO2 assisted process for the production of high-purity and sterile nano-hydroxyapatite/chitosan hybrid scaffolds.
Ruphuy, G; Souto-Lopes, M; Paiva, D; Costa, P; Rodrigues, A E; Monteiro, F J; Salgado, C L; Fernandes, M H; Lopes, J C; Dias, M M; Barreiro, M F.
Afiliación
  • Ruphuy G; Laboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials LSRE-LCM, Faculty of Engineering, University of Porto, Portugal.
  • Souto-Lopes M; Laboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials LSRE-LCM, Bragança Polytechnic Institute, Bragança, Portugal.
  • Paiva D; INEB-Institute of Biomedical Engineering, University of Porto, Portugal.
  • Costa P; Department of Metallurgical and Materials Engineering, Faculty of Engineering of the University of Porto, Portugal.
  • Rodrigues AE; i3S-Instituto de Investigação e Inovação em Saúde, University of Porto, Portugal.
  • Monteiro FJ; Laboratory of Process Engineering, Environment, Biotechnology and Energy LEPABE, Faculty of Engineering, University of Porto, Portugal.
  • Salgado CL; Laboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials LSRE-LCM, Faculty of Engineering, University of Porto, Portugal.
  • Fernandes MH; Laboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials LSRE-LCM, Faculty of Engineering, University of Porto, Portugal.
  • Lopes JC; INEB-Institute of Biomedical Engineering, University of Porto, Portugal.
  • Dias MM; Department of Metallurgical and Materials Engineering, Faculty of Engineering of the University of Porto, Portugal.
  • Barreiro MF; i3S-Instituto de Investigação e Inovação em Saúde, University of Porto, Portugal.
J Biomed Mater Res B Appl Biomater ; 106(3): 965-975, 2018 04.
Article en En | MEDLINE | ID: mdl-28470936
Hybrid scaffolds composed of hydroxyapatite (HAp), in particular in its nanometric form (n-HAp), and chitosan (CS) are promising materials for non-load-bearing bone graft applications. The main constraints of their production concern the successful implementation of the final purification/neutralization and sterilization steps. Often, the used purification strategies can compromise scaffold structural features, and conventional sterilization techniques can result in material's thermal degradation and/or contamination with toxic residues. In this context, this work presents a process to produce n-HAp/CS scaffolds mimicking bone composition and structure, where an innovative single step based on supercritical CO2 extraction was used for both purification and sterilization. A removal of 80% of the residual acetic acid was obtained (T = 75°C, p = 8.0 MPa, 2 extraction cycles of 2 h) giving rise to scaffolds exhibiting adequate interconnected porous structure, fast swelling and storage modulus compatible with non-load-bearing applications. Moreover, the obtained scaffolds showed cytocompatibility and osteoconductivity without further need of disinfection/sterilization procedures. Among the main advantages, the proposed process comprises only three steps (n-HAp/CS dispersion preparation; freeze-drying; and supercritical CO2 extraction), and the supercritical CO2 extraction show clear advantages over currently used procedures based on neutralization steps. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 965-975, 2018.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dióxido de Carbono / Durapatita / Quitosano / Nanoestructuras / Andamios del Tejido Límite: Humans Idioma: En Revista: J Biomed Mater Res B Appl Biomater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2018 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dióxido de Carbono / Durapatita / Quitosano / Nanoestructuras / Andamios del Tejido Límite: Humans Idioma: En Revista: J Biomed Mater Res B Appl Biomater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2018 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Estados Unidos