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Comparison of bacterial attachment to platelet bags with and without preconditioning with plasma.
Loza-Correa, M; Kalab, M; Yi, Q-L; Eltringham-Smith, L J; Sheffield, W P; Ramirez-Arcos, S.
Afiliación
  • Loza-Correa M; Canadian Blood Services, Centre for Innovation, Ottawa, ON, Canada.
  • Kalab M; Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
  • Yi QL; Canadian Blood Services, Centre for Innovation, Ottawa, ON, Canada.
  • Eltringham-Smith LJ; Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
  • Sheffield WP; Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
  • Ramirez-Arcos S; Canadian Blood Services, Centre for Innovation, Hamilton, ON, Canada.
Vox Sang ; 112(5): 401-407, 2017 Jul.
Article en En | MEDLINE | ID: mdl-28466544
BACKGROUND AND OBJECTIVES: Canadian Blood Services produces apheresis and buffy coat pooled platelet concentrates (PCs) stored in bags produced by two different manufacturers (A and B, respectively), both made of polyvinyl chloride-butyryl trihexyl citrate. This study was aimed at comparing Staphylococcus epidermidis adhesion to the inner surface of both bag types in the presence or absence of plasma factors. MATERIALS AND METHODS: Sets (N = 2-6) of bags type A and B were left non-coated (control) or preconditioned with platelet-rich, platelet-poor or defibrinated plasma (PRP, PPP and DefibPPP, respectively). Each bag was inoculated with a 200-ml S. epidermidis culture adjusted to 0·5 colony-forming units/ml. Bags were incubated under platelet storage conditions for 7 days. After culture removal, bacteria attached to the plastic surface were either dislodged by sonication for bacterial quantification or examined in situ by scanning electron microscopy (SEM). RESULTS: Higher bacterial adhesion was observed to preconditioned PC bags than control containers for both bag types (P < 0·0001). Bacterial attachment to preconditioned bags was confirmed by SEM. Bacteria adhered equally to both types of containers in the presence of PRP, PPP and DefibPPP residues (P > 0·05). By contrast, a significant increase in bacterial adherence was observed to type A bags compared with type B bags in the absence of plasma (P < 0·05) [Correction added on 16 June 2017, after first online publication: this sentence has been corrected]. CONCLUSION: The ability of S. epidermidis to adhere to preconditioned platelet collection bags depends on the presence of plasma factors. Future efforts should be focused on reducing plasma proteins' attachment to platelet storage containers to decrease subsequent bacterial adhesion.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus epidermidis / Plaquetas / Conservación de la Sangre / Incrustaciones Biológicas Límite: Humans Idioma: En Revista: Vox Sang Año: 2017 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus epidermidis / Plaquetas / Conservación de la Sangre / Incrustaciones Biológicas Límite: Humans Idioma: En Revista: Vox Sang Año: 2017 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido