Virus removal or inactivation in hemoglobin solutions by ultrafiltration or detergent/solvent treatment.
Biomater Artif Cells Artif Organs
; 16(1-3): 123-8, 1988.
Article
en En
| MEDLINE
| ID: mdl-2846096
Two procedures to eliminate virus infectivity from hemoglobin solutions at ambient temperature were evaluated. In the first, virus removal was assessed during the ultrafiltration of hemoglobin solutions through a membrane with a nominal molecular weight cut-off of 100,000 Daltons. The results of this study demonstrated that less than 0.1% of any virus originally spiked into the solution was detectable in the ultrafiltrate. In the second procedure the inactivation of viruses in hemoglobin solutions incubated with tri(n-butyl)phosphate mixed with sodium cholate was studied. Greater than 99% of each of the enveloped viruses tested was inactivated during the first 15 minutes of incubation with greater than 10(5) plaque forming units/ml of each being inactivated after one to six hours. No inactivation of the non-enveloped poliovirus was effected by this treatment. The data imply that both ultrafiltration and detergent/solvent incubation may reduce virus infectivity in hemoglobin solutions, but neither method yields a completely virus free product.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos Organofosforados
/
Organofosfatos
/
Virus
/
Hemoglobinas
/
Ultrafiltración
/
Ácidos Cólicos
Idioma:
En
Revista:
Biomater Artif Cells Artif Organs
Asunto de la revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
/
TRANSPLANTE
Año:
1988
Tipo del documento:
Article
Pais de publicación:
Estados Unidos