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Redox Changes During the Cell Cycle in the Embryonic Root Meristem of Arabidopsis thaliana.
de Simone, Ambra; Hubbard, Rachel; de la Torre, Natanael Viñegra; Velappan, Yazhini; Wilson, Michael; Considine, Michael J; Soppe, Wim J J; Foyer, Christine H.
Afiliación
  • de Simone A; 1 Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds , Leeds, United Kingdom .
  • Hubbard R; 1 Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds , Leeds, United Kingdom .
  • de la Torre NV; 2 Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research , Cologne, Germany .
  • Velappan Y; 1 Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds , Leeds, United Kingdom .
  • Wilson M; 3 School of Agriculture and Environment, The University of Western Australia , Perth, Australia .
  • Considine MJ; 1 Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds , Leeds, United Kingdom .
  • Soppe WJJ; 1 Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds , Leeds, United Kingdom .
  • Foyer CH; 3 School of Agriculture and Environment, The University of Western Australia , Perth, Australia .
Antioxid Redox Signal ; 27(18): 1505-1519, 2017 Dec 20.
Article en En | MEDLINE | ID: mdl-28457165
AIMS: The aim of this study was to characterize redox changes in the nuclei and cytosol occurring during the mitotic cell cycle in the embryonic roots of germinating Arabidopsis seedlings, and to determine how redox cycling was modified in mutants with a decreased capacity for ascorbate synthesis. RESULTS: Using an in vivo reduction-oxidation (redox) reporter (roGFP2), we show that transient oxidation of the cytosol and the nuclei occurred at G1 in the synchronized dividing cells of the Arabidopsis root apical meristem, with reduction at G2 and mitosis. This redox cycle was absent from low ascorbate mutants in which nuclei were significantly more oxidized than controls. The cell cycle-dependent increase in nuclear size was impaired in the ascorbate-deficient mutants, which had fewer cells per unit area in the root proliferation zone. The transcript profile of the dry seeds and size of the imbibed seeds was strongly influenced by low ascorbate but germination, dormancy release and seed aging characteristics were unaffected. INNOVATION: These data demonstrate the presence of a redox cycle within the plant cell cycle and that the redox state of the nuclei is an important factor in cell cycle progression. CONCLUSIONS: Controlled oxidation is a key feature of the early stages of the plant cell cycle. However, sustained mild oxidation restricts nuclear functions and impairs progression through the cell cycle leading to fewer cells in the root apical meristem. Antioxid. Redox Signal. 27, 1505-1519.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxidación-Reducción / Proteínas de Plantas / Arabidopsis / Meristema Idioma: En Revista: Antioxid Redox Signal Asunto de la revista: METABOLISMO Año: 2017 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxidación-Reducción / Proteínas de Plantas / Arabidopsis / Meristema Idioma: En Revista: Antioxid Redox Signal Asunto de la revista: METABOLISMO Año: 2017 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos