Post-transcriptional regulation of FUS and EWS protein expression by miR-141 during neural differentiation.

Svetoni, Francesca; De Paola, Elisa; La Rosa, Piergiorgio; Mercatelli, Neri; Caporossi, Daniela; Sette, Claudio; Paronetto, Maria Paola

Svetoni, Francesca; De Paola, Elisa; La Rosa, Piergiorgio; Mercatelli, Neri; Caporossi, Daniela; Sette, Claudio; Paronetto, Maria Paola.

Afiliación

Svetoni F; Department of Movement, Human and Health Sciences, University of Rome "Foro Italico", 00135 Rome, Italy.
De Paola E; Laboratories of Cellular and Molecular Neurobiology and of Neuroembryology, Fondazione Santa Lucia, 00143 Rome, Italy.
La Rosa P; Department of Movement, Human and Health Sciences, University of Rome "Foro Italico", 00135 Rome, Italy.
Mercatelli N; Laboratories of Cellular and Molecular Neurobiology and of Neuroembryology, Fondazione Santa Lucia, 00143 Rome, Italy.
Caporossi D; Laboratories of Cellular and Molecular Neurobiology and of Neuroembryology, Fondazione Santa Lucia, 00143 Rome, Italy.
Sette C; Department of Biomedicine and Prevention, University of Rome "Tor Vergata", Rome, Italy.
Paronetto MP; Department of Movement, Human and Health Sciences, University of Rome "Foro Italico", 00135 Rome, Italy.

Hum Mol Genet ; 26(14): 2732-2746, 2017 07 15.

Article en En | MEDLINE | ID: mdl-28453628

RESUMEN

Brain development involves proliferation, migration and specification of neural progenitor cells, culminating in neuronal circuit formation. Mounting evidence indicates that improper regulation of RNA binding proteins (RBPs), including members of the FET (FUS, EWS, TAF15) family, results in defective cortical development and/or neurodegenerative disorders. However, in spite of their physiological relevance, the precise pattern of FET protein expression in developing neurons is largely unknown. Herein, we found that FUS, EWS and TAF15 expression is differentially regulated during brain development, both in time and in space. In particular, our study identifies a fine-tuned regulation of FUS and EWS during neuronal differentiation, whereas TAF15 appears to be more constitutively expressed. Mechanistically FUS and EWS protein expression is regulated at the post-transcriptional level during neuron differentiation and brain development. Moreover, we identified miR-141 as a key regulator of these FET proteins that modulate their expression levels in differentiating neuronal cells. Thus, our studies uncover a novel link between post-transcriptional regulation of FET proteins expression and neurogenesis.

Asunto(s)

MicroARNs/metabolismo; Neuronas/fisiología; Procesamiento Postranscripcional del ARN; Proteína EWS de Unión a ARN/biosíntesis; Proteína FUS de Unión a ARN/biosíntesis; Animales; Encéfalo/citología; Encéfalo/embriología; Encéfalo/metabolismo; Diferenciación Celular/fisiología; Humanos; Ratones; Ratones Endogámicos C57BL; MicroARNs/genética; Neurogénesis/fisiología; Neuronas/citología; Neuronas/metabolismo; Procesamiento Proteico-Postraduccional; Proteína EWS de Unión a ARN/genética; Proteína EWS de Unión a ARN/metabolismo; Proteína FUS de Unión a ARN/genética; Proteína FUS de Unión a ARN/metabolismo; Proteínas de Unión al ARN/metabolismo; Factores Asociados con la Proteína de Unión a TATA/biosíntesis; Factores Asociados con la Proteína de Unión a TATA/genética; Factores Asociados con la Proteína de Unión a TATA/metabolismo

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Procesamiento Postranscripcional del ARN / Proteína EWS de Unión a ARN / Proteína FUS de Unión a ARN / MicroARNs / Neuronas Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Hum Mol Genet Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA MEDICA Año: 2017 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido

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