Effect of docosahexanoic acid on quality of frozen-thawed bull semen in BioXcell extender.
Reprod Fertil Dev
; 29(3): 490-495, 2017 Mar.
Article
en En
| MEDLINE
| ID: mdl-28442061
This study was conducted to investigate the effect of docosahexanoic acid (DHA) supplementation in BioXcell extender on the quality of frozen-thawed bull semen. Twenty-four ejaculates were collected from three bulls (eight from each bull). Ejaculates with motility ≥70% and normal morphology ≥80% were extended into BioXcell extender to which 0 (control), 3, 5, 10 or 15ngmL-1 DHA was added. The supplemented semen samples were incubated at 37°C for 15min for DHA uptake by spermatozoa. Later, samples were cooled for 2h at 5°C and packaged into 0.25-mL straws, frozen in liquid nitrogen for 24h and subsequently thawed for evaluation. Results are presented as percentages ± s.e.m. Supplementation with DHA at 3ngmL-1 significantly improved sperm functional parameters including sperm motility, normal morphology, viability, acrosome integrity and membrane integrity when compared with other supplemented groups and the control. Lipid peroxidation increased as the incorporation of DHA supplementation increased. In conclusion, 3ngmL-1 concentration of DHA resulted in superior quality of frozen-thawed bull spermatozoa and is suggested as the optimum level of DHA to be added into BioXcell extender.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Semen
/
Preservación de Semen
/
Espermatozoides
/
Ácidos Docosahexaenoicos
/
Crioprotectores
Límite:
Animals
Idioma:
En
Revista:
Reprod Fertil Dev
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Malasia
Pais de publicación:
Australia