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Identification of target genes downstream of semaphorin6A/PlexinA2 signaling in zebrafish.
Emerson, Sarah E; St Clair, Riley M; Waldron, Ashley L; Bruno, Sierra R; Duong, Anna; Driscoll, Heather E; Ballif, Bryan A; McFarlane, Sarah; Ebert, Alicia M.
Afiliación
  • Emerson SE; Department of Biology, University of Vermont, Burlington, Vermont.
  • St Clair RM; Department of Biology, University of Vermont, Burlington, Vermont.
  • Waldron AL; Department of Biology, University of Vermont, Burlington, Vermont.
  • Bruno SR; Department of Biology, University of Vermont, Burlington, Vermont.
  • Duong A; Department of Biology, University of Vermont, Burlington, Vermont.
  • Driscoll HE; Vermont Genetics Network Bioinformatics Core and Department of Biology, Norwich University, Northfield, Vermont.
  • Ballif BA; Department of Biology, University of Vermont, Burlington, Vermont.
  • McFarlane S; Department of Cell Biology and Anatomy, Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada.
  • Ebert AM; Department of Biology, University of Vermont, Burlington, Vermont.
Dev Dyn ; 246(7): 539-549, 2017 07.
Article en En | MEDLINE | ID: mdl-28440030
BACKGROUND: Semaphorin (Sema)/Plexin (Plxn) signaling is important for many aspects of neuronal development, however, the transcriptional regulation imposed by this signaling pathway is unknown. Previously, we identified an essential role for Sema6A/PlxnA2 signaling in regulating proliferation and cohesion of retinal precursor cells (RPCs) during early eye development. This study used RNA isolated from control, Sema6A-deficient and PlxnA2-deficient zebrafish embryos in a microarray analysis to identify genes that were differentially expressed when this signaling pathway was disrupted. RESULTS: We uncovered a set of 58 transcripts, and all but 1 were up-regulated in both sema6A and plxnA2 morphants. We validated gene expression changes in subset of candidates that are suggested to be involved in proliferation, migration or neuronal positioning. We further functionally evaluated one gene, rasl11b, as contributing to disrupted proliferation in sema6A and plxna2 morphants. Our results suggest rasl11b negatively regulates proliferation of RPCs in the developing zebrafish eye. CONCLUSIONS: Microarray analysis has generated a resource of target genes downstream of Sema6A/PlxnA2 signaling, which can be further investigated to elucidate the downstream effects of this well-studied neuronal and vascular guidance signaling pathway. Developmental Dynamics 246:539-549, 2017. © 2017 Wiley Periodicals, Inc.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Receptores de Superficie Celular / Regulación del Desarrollo de la Expresión Génica / Proteínas de Pez Cebra / Semaforinas Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Dev Dyn Asunto de la revista: ANATOMIA Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Receptores de Superficie Celular / Regulación del Desarrollo de la Expresión Génica / Proteínas de Pez Cebra / Semaforinas Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Dev Dyn Asunto de la revista: ANATOMIA Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos