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A novel in vitro ELISA for estimation of glycoprotein content in human rabies vaccines.
Aavula, Shukra M; Abhinay, Gontu; Nimmagadda, Sridevi V; Maithal, Kapil.
Afiliación
  • Aavula SM; a Research and Development Centre , Indian Immunologicals Limited, Rakshapuram, Gachibowli , Hyderabad , Telangana , India.
  • Abhinay G; a Research and Development Centre , Indian Immunologicals Limited, Rakshapuram, Gachibowli , Hyderabad , Telangana , India.
  • Nimmagadda SV; a Research and Development Centre , Indian Immunologicals Limited, Rakshapuram, Gachibowli , Hyderabad , Telangana , India.
  • Maithal K; a Research and Development Centre , Indian Immunologicals Limited, Rakshapuram, Gachibowli , Hyderabad , Telangana , India.
J Immunoassay Immunochem ; 38(4): 400-410, 2017.
Article en En | MEDLINE | ID: mdl-28436708
In vitro methods for quantification of immunodominant glycoprotein in the rabies vaccine formulations serve as good alternative to the cumbersome and variable mice potency assay as a batch release test for the vaccine. The present study presents the development of a sandwich ELISA with optimal concentrations of a high affinity recombinant diabody (D06) and a specific monoclonal antibody (M5B4) against rabies glycoprotein for its quantification in the vaccine formulations. The glycoprotein estimate correlated linearly (r2 = 0.8) to the in vivo potency estimate for the vaccine formulations. This ELISA promises a good forecast of the mice potency values and thereby can serve as a simple, yet effective batch release test for the rabies vaccines replacing the in vivo assay.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ensayo de Inmunoadsorción Enzimática / Vacunas Antirrábicas / Glicoproteínas Límite: Animals / Humans Idioma: En Revista: J Immunoassay Immunochem Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ensayo de Inmunoadsorción Enzimática / Vacunas Antirrábicas / Glicoproteínas Límite: Animals / Humans Idioma: En Revista: J Immunoassay Immunochem Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido